新疆1816名艾滋病病毒抑制失败患者耐药分析  

Drug resistance of 1816 viral suppression failure cases of HIV treatment,Xinjiang

作　　者：王凤英[1] 金涛[1] 胡晓远[1] 倪明健[1] WANG Feng - ying;JIN Tao;HU Xiao - yuan;NI Ming - jian(The Xinjiang Uygur Autonomous Region Center for Disease Control and Prevention, Urumchi ,Xinjiang 830000, Chin)

机构地区：[1]新疆维吾尔自治区疾病预防控制中心,新疆乌鲁木齐830000

出　　处：《现代预防医学》2018年第13期2447-2450,共4页Modern Preventive Medicine

基　　金：国家科技重大专项(2013ZX10004907)

摘　　要：目的了解新疆抗病毒治疗病毒抑制失败患者对免费抗病毒治疗药物的耐药状况,并寻找可能导致的耐药因素,给予建议。方法选择HIV在治患者病毒载量大于1 000拷贝/ml的样本1 816份,用in-house套式聚合酶链反应扩增HIV pol区基因,通过序列测定,用ChromasP ro软件进行序列拼接分析,进入Stanford HIV耐药数据库序列结果比对,获得耐药位点和耐药程度分析结果。结果 1 816例患者中有916例对至少一种药物产生耐药,占50.44%,南疆四地州的蛋白酶抑制剂类药物耐药率比较突出,达到4%～5%,但3类药物当中仍然是非核苷类药物耐药率最高,达到43.61%。并未出现特殊的耐药突变位点。结论新疆艾滋病病毒抑制失败患者中耐药突变率较高,在艾滋病疫情发展较快的南疆四地州出现了蛋白酶抑制剂类药物的耐药,需要引起关注。Objective To understand the status of drug resistance of HIV antiviral therapy in Xinjiang,and to look for possible resistance factors. Methods 1 816 samples of HIV in patients with viral load of more than 1000 copies/ml were selected for HIV-1 genotypic resistance assay,pol gene was amplified by using nested-pcr of in-house,sequence splicing was analyzed using Chromas Pro,then the analysis results of drug resistance were acquired in Stanford HIV drug resistance database. Results Of the 1 816 patients,916 were resistant to at least one drug,accounting for 50. 44%. The drug resistance of PI was outstanding in the four prefectures of southern Xinjiang,reaching to 4-5%,but the drug resistance of NNRTI was the highest in three types of drugs,reaching to 43. 61%. There was no specific drug resistance mutation site. Conclusion The drug resistance mutation rate was higher in the virological failure of HIV in Xinjiang. The rapid development of HIV in southern Xinjiang has developed a protease inhibitor drug resistance.

关 键 词：新疆 艾滋病 耐药 

分 类 号：R183[医药卫生—流行病学]