电针预处理对全脑缺血小鼠海马组织微RNA-223的影响  

Regulatory effects of electroacupuncture pretreatment on microRNA-223 in the hippocampus of mice with global cerebral ischemia injury

作　　者：刘曌宇 朱萧玲 杨谦梓 Liu Zhaoyu;Zhu Xiaoling;Yang Qianzi(Department of Anesthesiology, Xijing Hospital, Air Force Medical University, Xi＇ an 710032, China)

机构地区：[1]空军军医大学西京医院麻醉科,西安710032

出　　处：《国际麻醉学与复苏杂志》2018年第6期527-530,544,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金（81503373）;国家重点基础研究发展计划（973计划）（2014CB543202）

摘　　要：目的探索电针（electroacupuncture, EA）预处理对全脑缺血（global cerebral ischemia, GCI）损伤后小鼠海马神经元微RNA-223（microRNA-223, miR-223）表达的影响，探讨miR-223在EA预处理诱导的脑缺血耐受中的作用。方法采用随机数字表法将25只成年雄性C57/BL小鼠分为假手术组（Sham组，5只）、GCI组（15只）、EA预处理组（EA＋GCI组，5只）。Sham组小鼠只暴露手术部位组织，不做缺血处理。GCI组制作短暂性GCI模型，分别于再灌注后12、24、36 h处死小鼠取脑。EA＋GCI组选取小鼠百会穴EA刺激30 min，2 h后同GCI组制作GCI模型。实时荧光定量聚合酶链式反应（real-time fluorescence quantitative PCR, real-time fqPCR）检测小鼠海马组织miR-223的表达变化；再灌注后24 h行神经功能学评分、尼氏（Nissl）染色检测海马组织CA1区椎体神经元存活情况。结果与Sham组比较，GCI组小鼠再灌注后24 h海马组织miR-223表达明显增多（P〈0.05）；与GCI组比较，EA＋GCI组小鼠再灌注后24 h海马组织miR-223的表达下降（P〈0.05），神经功能学评分明显提高（P〈0.05），CA1区锥体神经元存活数目增多（P〈0.05）。结论GCI损伤可使小鼠脑内miR-223表达增多，而EA预处理诱导的脑保护作用可能与其抑制miR-223的表达有关。ObjectiveTo investigate whether electroacupuncture（EA） pretreatment affects the expression of microRNA-223 （miR-223） in the hippocampus of mice with global cerebral ischemia （GCI） injury.MethodsA total of twenty-five adult male C57/BL mice were randomly divided into three groups according to the random numbers table method： Sham group（n=5）, GCI group （n=15）, EA pretreatment group（EA＋GCI group, n=5）. In the sham group, a ventral midline incision was made to expose the trachea without any other procedures. Mice in GCI group were subjected to global cerebral ischemia, animals were sacrificed and the hippocampus was dissected at 12, 24, 36 h after reperfusion, respectively. In the EA＋GCI group, mice were given EA pretreatment at Baihui acupoint for 2 h before GCI. The miR-223 expression in the hippocampus was assayed with real-time fluorescence quantitative PCR（real-time fqPCR） after GCI-reperfusion injury. Neurobehavioral evaluation and Nissl staining were used to analyze the neuroprotection by EA pretreatment.ResultsCompared with the Sham group, there was a marked increase in miR-223 expression in the pyramidal neurons 24 h after reperfusion（P〈0.05）. EA pretreatment significantly blocked the up-regulation of miR-223 and improved the neurological scores compared with GCI group （P〈0.05）. There was also a significant increase in the viability of CA1 pyramidal neurons in EA＋GCI group, compared with that of the GCI group（P〈0.05）.ConclusionsEA preconditioning improves cerebral ischemia tolerance after GCI via suppressing the up-regulation of miR-223.

关 键 词：微RNA 全脑缺血 电针刺 预处理 

分 类 号：R245[医药卫生—针灸推拿学]