工作场所空气中对氨基苯甲醚检测的高效液相色谱法  

Determination of p-Anisidine in workplace air by HPLC

作　　者：姜开友 秦文华 耿琪 孟成名 余善法 Jiang Kaiyou;Qin Wenhaa;Gen Qi;Meng Chengming;Yu Shanfa(Henan Provincial Institute of Occupational Health, Zhengzhou 450052, Chin)

机构地区：[1]河南省职业病防治研究院,郑州450052

出　　处：《中华劳动卫生职业病杂志》2018年第6期468-471,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的建立工作场所空气中对氨基苯甲醚的高效液相色谱检测法。方法采用高效液相色谱法，色谱柱为C18柱，流动相为甲醇：纯水=62：38（v／v），流速0．9ml／min，检测波长240nm，进样体积20μl。结果该方法测定对氨基苯甲醚线性范围（pg／m1）为0~100；相关系数（r）0．9997；最低检出限为0．15wg／ml；平均精密度RSD为4．4％。以硅胶管为采样滤料，采样效率〉95％。样品室温下可保存7d以上。结论该方法采样效率高，操作简便，精密度和灵敏度较高，可以满足工作场所空气中对氨基苯甲醚的测定。Objective To establish an HPLC method for determining p-Anisidine in workplace air. Methods An C18 capillary column was used with the detection wavelength of 240 nm, the methanol solution （v/v=62：38） as mobile phase, the flow rate was 0.9 ml/min, and the injection volume was 20μl. Results The linear range （μg/ml） for p-Anisidine was 0-100; correlation coefficient was 0.999 7; the detection limit was 0.15 μg/ml; the average precision of RSD was 4.4%. The sampling efficiency was more than 95% using silicone tube as sampling filter. Sample can be stored at room temperature for more than 7 d. Conclusion the method appered to be accurate, simple, sensitive and reliable, also have a high sampling efficiency, and can be usd to determine p-anisidine in workplace air simultaneously.

关 键 词：色谱法 高效液相 工作场所 对氨基苯甲醚 

分 类 号：O657.72[理学—分析化学] R134.4[理学—化学]