金水宝胶囊对大鼠尘肺模型的药效学研究  

Pharmacodynamic study on Jinshuibao Capsules on rat pneumoconiosis model

作　　者：王静 蒋舒凡 胡伟燚 涂雷 王涛[2] 刘薇 WANG Jing;JIA NG Shu-fan;H U Wei-yi;TU Lei;WANG Tao;LIU Wei(Pharmacy Department, Nanjing Prevention and Treatment Center for Occupational Diseases, Nanjing Jiangsu, 210042, China;Ne w Drug Sc ree ning Ce nte r, China Pharmaceutic al Unive rs ity , Nanjing Jiangsu, 210009, China)

机构地区：[1]南京市职业病防治院药剂科,江苏南京210042 [2]中国药科大学新药筛选中心,江苏南京210009

出　　处：《职业与健康》2018年第13期1772-1776,共5页Occupation and Health

基　　金：2015年度南京市医学科技发展项目(YKK15190)

摘　　要：目的考察金水宝胶囊对大鼠尘肺模型呼吸功能和肺组织病变的改善作用。方法通过气管注射石英粉尘建立大鼠慢性尘肺模型。造模成功后,将大鼠随机分为模型组、汉防己甲素片组、金水宝胶囊600 mg/kg组和金水宝胶囊300 mg/kg组,每组24只,另取24只正常大鼠作为空白对照组。各组大鼠灌胃给药。每天1次,每周6天,连续6个月。试验期间连续观察一般状况及体重测定,给药的第1、2、3、6个月,各组分别取6只大鼠,进行呼吸功能、肺动脉压力、血气分析、肺组织重量、氧化应激和羟脯氨酸(HP)水平等药效学检测。结果 6个月给药期间,金水宝胶囊组大鼠一般状态和体重未见明显异常。金水宝胶囊高、低剂量组均能降低尘肺模型大鼠呼吸频率并增加呼吸幅度(均P<0.05),同时显著改善血气指标和低血氧症状,包括升高pH值(金水宝胶囊高、低剂量组、模型组分别为7.15~7.22、7.13~7.20和6.93~7.10),降低PaCO_2(金水宝胶囊高、低剂量组、模型组分别为40.1~41.9、40.6~43.1和47.2~49.0 mm Hg)、升高PaO_2(金水宝胶囊高、低剂量组、模型组分别为87.0~88.9、86.0~87.9和80.6~83.0 mm Hg)和Sa O2(金水宝胶囊高、低剂量组、模型组分别为92.5%~94.2%、91.5%~93.8%和83.5%~87.7%)(均P<0.05,)。在6个月试验期间,金水宝胶囊高、低剂量组均能显著降低模型大鼠的平均肺动脉压(均P<0.05)。金水宝胶囊高、低剂量组对于模型大鼠1、2、3、6个月肺重量系数均有显著的降低作用(均P<0.05),给药2个月后亦可以明显降低肺组织HP含量,高剂量组对于模型大鼠1、2、3、6个月丙二醇(MDA)水平和超氧化物歧化酶(SOD)活力均有显著的改善作用(均P<0.05)。结论金水宝胶囊对于大鼠尘肺模型的呼吸功能和肺组织病变具有较好的改善作用,药效作用具有一定的时间和剂量依赖性。[Objective]To investigate the improvement effect of Jinshuibao Capsules on respiratory function and lung tissue lesion in rat pneumoconiosis model.[Methods]The rat model of chronic pneumoconiosis was established by tracheal injection of quartz dust. After successful modeling,rats were randomly divided into the model group and Tetrandrine Tablets group,600 mg/kg Jinshuibao Capsules group,and 300 mg/kg Jinshuibao Capsules group,24 rats in each group. Meanwhile,24 normal rats were included in a blank control group. Rats in each group were treated by intragastric administration,once a day,6 days a week for6 months. During the experiment,the general condition and body weight were observed continuously. At the first,second,third and sixth month of administration,6 rats in each group were taken for detecting respiratory function,pulmonary artery pressure,blood gas analysis,lung tissue weight,oxidative stress and hydroxyproline（HP） level. [Results] During 6 months＇ administration,there was no obvious abnormality in the general condition and body weight of rats in treatment groups. Both high and low dose Jinshuibao Capsules could reduce the respiratory rate and increase the respiratory magnitude of pneumoconiosis rats（all P〈0.05）,and significantly improve the blood gas indexes and hypoxemia symptoms,including increasing the pH value（high dose Jinshuibao Capsules group,low dose Jinshuibao Capsules group and model group was respectively 7.15-7.22,7.13-7.20 and6.93-7.10）,decreasing PaCO_2 level（high dose Jinshuibao Capsules group,low dose Jinshuibao Capsules group and model group was respectively 40.1-41.9,40.6-43.1 and 47.2-49.0 mm Hg）,and elevating PaO_2 level（high dose Jinshuibao Capsules group,low dose Jinshuibao Capsules group and model group was respectively 87.0-88.9,86.0-87.9 and 80.6-83.0 mm Hg） and Sa O2 level（high dose Jinshuibao Capsules group,low dose Jinshuibao Capsules group and model group was respectively 92.5%-94.2%,91.5%-93.8% and 83.5%-87.7%）（all P〈0.05）. During 6 months�

关 键 词：金水宝胶囊 大鼠 尘肺 肺动脉压力 肺纤维化 

分 类 号：R332[医药卫生—人体生理学]