水稻种子特异表达基因OsEnS38的克隆与表达  

Cloning and expression analysis of a seed–specific gene OsEnS38 in rice

作　　者：张焕 仇忠凯 阎新[1,2] 欧阳解秀 李绍波[1,2] 王鑫 ZHANG Huan;QIU Zhongkai;YAN Kin;OUYANG Jiexiu;LI Shaobo;WANG Kin(College of Life Sciences, Nanchang University, Nanchang, Jiangxi 330031,China;Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, Nanchang, Jiangxi 330031,China)

机构地区：[1]南昌大学生命科学学院,江西南昌330031 [2]江西省分子生物学与基因工程重点实验室,江西南昌330031

出　　处：《湖南农业大学学报（自然科学版）》2018年第3期278-283,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31760080;31560298)

摘　　要：以粳稻中花11为材料,利用生物信息学技术,结合RT–PCR,克隆了水稻OsEnS38的c DNA序列;其编码的蛋白序列包含2个Cupin_1保守结构域,属于典型的Bicupin亚家族。启动子分析发现,OsEnS38启动子区域含有多种与胚乳特异表达相关的顺式作用元件;q RT–PCR和原位杂交结果证实,OsEnS38在开花后7~14 d的种子中特异表达,在第10天的胚乳中表达量最高,达29.73;杂交信号在第7、10天的胚乳中高表达;共表达分析显示,共表达基因参与水稻胚后发育和生殖发育调控,表明该基因在种子发育和储藏物质积累过程中发挥作用。In this study, we identified and cloned the OsEnS38 c DNA sequence in Zhonghua 11 using bioinformatic and RT–PCR analysis. The encoded OsEnS38 contains two conservative cupin_1 domain typical in Bicupin superfamily. Promoter sequence analysis revealed the existence of several confirmed endosperm–specific cis–regulatory elements in the OsEnS38 promoter. It was confirmed by q RT–PCR and in situ hybridization analysis that the expression of OsEnS38 was specifically expression in developing seeds 7–14 days after pollination. Further analysis revealed that OsEnS38 together with its co–expressing genes were liable to participate in post–embryonic development and reproduction. The results suggested that it might play a role in seed development and accumulation of storage.

关 键 词：水稻 种子特异表达基因 基因克隆 原位杂交 共表达分析 

分 类 号：S511.035.3[农业科学—作物学]