日本结缕草ZjLEA3基因克隆、亚细胞定位及表达分析  

Cloning, Subcellular Localization and Expression Analysis of ZjLEA3 Gene in Zoysia japonica

作　　者：刘海金[1] 滕菲 靳飞[2] 杨青青[2] 罗红松[3] Liu Haijin;Teng Fei;Jin Fei;Yang Qingqing;Luo Hongsong(Affiliated Middle School of Beijing College of Education,Beijing ,100035;Beijing Institute of Education,Beijing, 100011;Beijing Forestry University, Beijing, 100083)

机构地区：[1]北京教育学院附属中学,北京100035 [2]北京教育学院,北京100011 [3]北京林业大学,北京100083

出　　处：《分子植物育种》2018年第13期4219-4225,共7页Molecular Plant Breeding

摘　　要：为了研究日本结缕草抗逆机制,本研究以日本结缕草(Zoysia japonica)转录组的数据为基础,克隆得到ZjLEA3基因,其开放阅读框为555 bp,编码184个氨基酸。ZjLEA3基因包含LEA基因族保守区域,与其他植物LEA基因相似度在65.79%以上。软件分析表明该蛋白属于小分子亲水性蛋白;亚细胞定位结果显示该基因编码的蛋白定位于细胞质和细胞核中;ZjLEA3表达分析表明,该基因是受到了高温、高盐、PEG的诱导,由此推断ZjLEA3可能参与非生物胁迫的防御。本试验克隆并分析ZjLEA3基因是为日本结缕草抗逆机理研究提供帮助。In order to investigate the resistance mechanism of Zoysia japonica, ZjLEA3 gene was cloned based on the data from the Zoysia japonica transcriptome. Its open reading frame was 555 bp and encoded 184 amino acids.The ZjLEA3 gene contained a conserved region of the LEA gene family with a similarity of 65.79% or more with other plant LEA genes. Software analysis showed that the protein belonged to the small molecule hydrophilic protein; subcellular localization results indicated that the protein encoded by the gene was located in the cytoplasm and nucleus. ZjLEA3 expression analysis implied that the gene was induced by high temperature, high salt and polyethylene glycol（PEG）, suggesting that ZjLEA3 might be involved in abiotic stress defense. This study cloned and analyzed ZjLEA3 gene to provide a theoretical basis for the study on Zoysia japonica resistance mechanism.

关 键 词：日本结缕草 LEA基因 克隆 亚细胞定位 表达分析 

分 类 号：S688.4[农业科学—观赏园艺]