机械张力加载时间对成纤维细胞机械传导效应影响  

EFFECT OF TIME UNDER MECHANICAL TENSION ON MECHANOTRANSDUCTION OF FIBROBLASTS

作　　者：朱明宇[1] 李雨珊 王志国[1] 匡瑞霞[1] ZHU Mingyu;LI Yushan;WANG Zhiguo;KUANG Ruizia(Department of Plastic Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266003, Chin)

机构地区：[1]青岛大学附属医院整形美容科,山东青岛266003 [2]安阳市人民医院整形美容外科

出　　处：《青岛大学学报（医学版）》2018年第3期313-316,320,共5页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金项目(ZR2013HQ006);青岛市基础研究计划项目(13-1-4-161-jch)

摘　　要：目的探讨加载机械张力参数为拉伸幅度的10%时,机械张力的加载时间对正常皮肤成纤维细胞(NSFB)机械传导效应的影响。方法体外培养人体NSFB和增生性瘢痕成纤维细胞(HSFB),NSFB和HSFB分别设实验组和对照组,实验组加载机械张力参数为拉伸幅度10%,对照组不加载机械张力。在加载周期性机械张力3、5、7d时,用CCK-8检测各组细胞增殖能力,用Real-time PCR法检测各组细胞Integrin-β_1、P130Cas基因表达水平,用Western-Blot方法检测细胞Integrin-β_1、P130Cas蛋白表达水平。结果加载机械张力5d时,NSFB实验组与HSFB对照组比较,细胞增殖、P130Cas和Integrin-β_1的表达水平差异均无统计学意义(Z=-0.218,F=0.591、0.079,P>0.05);加载3、7d时,NSFB实验组与HSFB对照组比较,细胞增殖、P130Cas、Integrin-β_1表达差异均有统计学意义(Z=-5.294^-2.411,F=34.182~872.148,P<0.05)。结论拉伸幅度为10%的机械张力加载5d时,可使NSFB表现出与HSFB相同的机械传导效应。Objective To investigate the effect of time under mechanical tension（TUMT）on the mechanotransduction of normal skin fibroblasts（NSFB）when mechanical tension was set to 10%stretch amplitude. Methods Human NSFB and hypertrophic scar fibroblasts（HSFB）were cultured in vitro as test group and control group,respectively.The test group was loaded with mechanical tension of 10% stretch amplitude,while the control group was loaded with zero tension.When cyclic TUMT reached 3,5,and 7 d,cell proliferation in each group was determined by CCK-8 assay,while the mRNA and protein expression of Integrin-β1 and P130 Cas in each group were measured by real-time PCR and Western blot,respectively. Results When TUMT was 5 d,there were no significant differences between the test group and the control group in cell proliferation and the expression of Integrin-β1 and P130 Cas（Z=-0.218,F=0.591 and 0.079,P〉0.05）;but when TUMT was 3 or 7 d,there were significant differences between the test group and the control group in cell proliferation and the expression of Integrin-β1 and P130 Cas（Z=-5.294 to-2.411,F=34.182-872.148,P〈0.05）. Conclusion When TUMT of 10%stretch amplitude is 5 d,NSFB may exhibit the same mechanotransduction as HSFB.

关 键 词：成纤维细胞 瘢痕 皮肤 机械感受器 整合素类 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学] R339.19[医药卫生—基础医学]