TRAIL及其受体在PCOS病人卵巢颗粒细胞表达  

THE EXPRESSION OF TRAIL AND ITS RECEPTORS IN OVARIAN GRANULOSA CELLS IN PATIENTS WITH POLYCYSTIC OVARY SYNDROME

作　　者：李科 祁秀娟[1] 吕映频[1] 谭慧芳 苗佳 LI Ke;QI Xiujuan;LU Yingpin;TAN Huifang;MIAO Jia(Qingdao University Medical College, Qingdao 266071, China)

机构地区：[1]青岛大学附属医院生殖医学科,山东青岛266071 [2]青岛大学医学部

出　　处：《青岛大学学报（医学版）》2018年第2期197-201,205,共6页Journal of Qingdao University(Medical Sciences)

摘　　要：目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体死亡受体4(DR4)和诱骗受体1(DcR1)在多囊卵巢综合征(PCOS)病人卵巢颗粒细胞的表达及其与PCOS发病的关系。方法收集PCOS病人30例及卵巢正常者(对照组)30例的卵巢颗粒细胞,RT-PCR方法分析两组TRAIL及其受体DR4、DcR1 mRNA表达情况,Western blot法检测两组TRAIL及其受体DR4、DcR1蛋白表达情况。结果 PCOS组TRAIL mRNA及蛋白表达较对照组明显增加(t=2.641、2.891,P<0.01),DcR1mRNA和蛋白表达较对照组明显降低(t=2.039、3.079,P<0.01),两组DR4mRNA和蛋白表达比较差异无显著性(P>0.05)。结论 PCOS病人卵巢颗粒细胞TRAIL及其受体异常表达,可能通过参与卵巢颗粒细胞凋亡调控导致PCOS发病。Objective To investigate the expression of tumor necrosis factor-related apoptosis-inducing ligand（TRAIL）and its receptors,death receptor 4（DR4）and decoy receptor 1（DcR1）,in ovarian granulosa cells as well as its correlation with the development of polycystic ovary syndrome（PCOS）in patients with PCOS. Methods Ovarian granulosa cells were collected from 30 PCOS patients and 30 healthy subjects（control group）.RT-PCR was used to analyze the expression of TRAILand its receptors DR4 and DcR1 in both groups.Western blot assay was employed to examine the protein expression of TRAIL,DR4,and DcR1 in both groups. Results RT-PCR and Western blot results showed that the mRNA and protein expression of TRAIL in the PCOS group was significantly stronger than that in the control group（t=2.641 and 2.891,P〈0.01）;the mRNA and protein expression of DcR1 was significantly weaker in the PCOS group than in the control group（t=2.039 and 3.079,P〈0.01）;no significant difference was observed between the two groups regarding the mRNA and protein expression of DR4（P〉0.05）. Conclusion The abnormal expression of TRAIL and its receptors in ovarian granulosa cells in PCOS patients may result in the development of PCOS by participating in the apoptosis regulation of ovarian granulosa cells.

关 键 词：多囊卵巢综合征 卵巢颗粒细胞 细胞凋亡 肿瘤坏死因子相关凋亡诱导配体 死亡受体4 受体 肿瘤坏死因子 成员10c 

分 类 号：R711.75[医药卫生—妇产科学]