餐厨垃圾和市政污泥配料比对厌氧产甲烷特性及微生物多样性的影响  

Biomethane production and microbial community change during the co-digestion of food waste and municipal sludge with different mix ratios

作　　者：朱英东 周俊[1,2] 雍晓雨[1,2] 谢欣欣[1,2] 仉丽娟[1,2] 贾红华[1,2] 韦萍 ZHU Yingdong;ZHOU Jun;YONG Xiaoyu;XIE Xinxin;ZHANG Lijuan;JIA Honghua;WEI Ping(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China;Bioenergy Research Institute,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800 [2]南京工业大学生物能源研究所,江苏南京211800

出　　处：《生物加工过程》2018年第3期60-70,共11页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金(21777069);国家重点研发计划重点专项课题(2016YFE0112800);江苏省重点研发计划(BE2016389)

摘　　要：在(38±0.5)℃的温度下,研究不同配料比对餐厨垃圾和市政污泥混合厌氧消化过程中系统参数及微生物群落多样性的影响,设定餐厨垃圾和市政污泥的挥发性固体的质量比为1∶0、0∶1、1∶1、1∶2和2∶1,并采用高通量测序技术分析厌氧消化过程中微生物组成的多样性。结果表明:质量比为1∶1的体系混合厌氧消化时,产甲烷效果最佳,单位质量的挥发性固体含量的产甲烷量可达到232.86 L/kg,挥发性固体含量的去除率达46.09%。各个混合比例体系中的优势物种为拟杆菌门(Bacteroidetes)和甲烷八叠球菌属(Methanosarcina)。在餐厨垃圾中添加市政污泥混合厌氧消化能够提高微生物群落的多样性,促进菌种间的协同作用,进而提升产气效率。In order to investigate the effects of food waste（ FW）/municipal sludge（ MS） ratio on the reactor performance and microbial community diversity at the mesophilic temperature（ 38 ± 0. 5） ℃,labscale experiments were carried out at five volatile solid（ VS） ratios of FW ∶ MS at 1 ∶ 0,0 ∶ 1,1 ∶ 1,1 ∶ 2,and 2 ∶ 1. High-throughput sequencing technique was also used to reveal the microbial community composition of the anaerobic digestion system. Results showed that food waste（ FW）/municipal sludge（ MS） ratio had complex influences on methane yield and microbial community. The accumulative maximum methane yield（ VS） 232. 86 L/kg and removal of volatile solid content（ 46. 09%） wereobtained when food waste（ FW）/municipal sludge（ MS） ratio was controlled at 1 ∶ 1. High-throughput sequencing indicated that Bacteroidetes and Methanosarcina dominated in whole process of all the mixed systems. In conclusion,co-digestion system could significantly improve methane production by increasing microbial community diversity and synergistic effects between different species.

关 键 词：厌氧 餐厨垃圾 市政污泥 甲烷 微生物群落 

分 类 号：X705[环境科学与工程—环境工程]