负载纳米氧化锌丝素胶原蛋白支架修复皮肤创面  

Acceleration of wound healing by a porous collagen/silk fibroin scaffold carrying zinc oxide nanoparticles

作　　者：李青[1] 全仁夫[1] 陈利红[1] 何丽红[1] 陈恩良[1] 胡华辉[1] 郑宣[1] Li Qing;Quan Ren-fu;Chen Li-hong;He Li-hong;Chen En-liang;Hu Hua-hui;Zheng Xuan(Research Institute of Orthopedics, Xiaoshan Traditional Chinese Medical Hospital, Hangzhou 311201, Zhejiang Province, China)

机构地区：[1]杭州市萧山区中医院,浙江中医药大学附属江南医院,浙江省杭州市311201

出　　处：《中国组织工程研究》2018年第14期2157-2161,共5页Chinese Journal of Tissue Engineering Research

基　　金：浙江省中医药科技计划科研基金项目(2015ZA173); 浙江省医药卫生科技计划项目(2015126904); 杭州市科技计划引导项目(20163501Y77); 萧山区重大科技专项(2015204)

摘　　要：背景:抗感染能力对于皮肤移植物的成败具有关键作用,将抗菌材料复合于支架上得到复合材料,是目前常用的增强移植物抗感染能力的方法之一。目的:研究负载纳米氧化锌丝素胶原蛋白支架的抗感染与抗炎作用潜能,以及其对创面修复再生的作用。方法:取32只SD大鼠,在其背部建立全层皮肤缺损模型,随机分2组干预,实验组植入负载纳米氧化锌颗粒的丝素胶原蛋白支架,对照组植入丝素胶原蛋白支架。移植后1,2,4,8周,测量创面剩余面积,计算创面愈合率;移植后1,2,4周,取创面组织,进行苏木精-伊红染色与白细胞介素6特异染色,观察创面组织形态与炎症反应,同时采用实时定量PCR检测创面组织炎症因子白细胞介素6、白细胞介素1β基因表达。结果与结论:(1)创面愈合率:实验组移植后2,4,8周的创面愈合率显著高于对照组(P<0.05);(2)苏木精-伊红染色:移植后1周,对照组有明显炎症细胞浸润;实验组炎症反应较轻,创面肉芽组织生长旺盛。移植后4周,实验组创面表皮组织结构完整而致密,基本完成创面修复;对照组未见表皮覆盖,为瘢痕化修复;(3)白细胞介素6特异染色:实验组与对照组白细胞介素6阳性着色,但程度深浅不一,移植后4周,对照组白细胞介素6表达量虽明显减少,但仍呈强阳性表达;实验组白细胞介素6呈弱阳性表达;(4)炎症因子表达:实验组移植后1,2,4周的白细胞介素6、白细胞介素1β基因表达均低于对照组,但仅移植后1,2周的组间比较差异有显著性意义(P<0.05);(5)结果表明:负载纳米氧化锌颗粒的丝素胶原蛋白支架移植,能有效减轻皮肤创伤后引起的炎症反应,加速完成皮肤创面修复。BACKGROUND： Anti-infective ability determine the success or failure of skin grafting. It is one of the commonly used methods to enhance the anti-infective ability of implants by compounding antibacterial materialswith scaffolds. OBJECTIVE： To investigate the effect of porous collagen/silk fibroin scaffolds carrying zinc oxide nanoparticles against infection and inflammation, and to evaluate its effect on wound healing. METHODS： Thirty-two Sprague-Dawley rats with a full-thickness wound on the back skin were randomly divided into two groups. In experimental groiup, porous collagen/silk fibroin scaffolds containing zinc oxide nanoparticles were implanted, while only collagen/silk fibroin scaffolds were implanted in control group. Wound healing was compared between the two groups by measuring residual wound area at 1, 2, 4, 8 weeks post implantation. Hematoxylin-eosin and interleukin 6 immumohistochemical staining were performed at 1, 2, 4 weeks post implantation to observe wound morphology and inflammatory reactions. Meanwhile, expression of interleukin 6 and interleukin 1β was detected by real-time PCR. RESULTS AND CONCLUSION：（1） At 2, 4, 8 weeks post implantation, significantly increased healing rate was observed in the experiment group compared with the control group（P〈0.05）.（2） Findings from the hematoxylin-eosin staining showed that obvious inflammatory cell infiltration was observed in the control group, but less inflammation with vigorous growth of granulation tissues on the wound surface occurred in the experimental group at 1 week after implantation. Then, the wound repair was basically completed in the experimental group presenting with complete and compact epidermal tissue structure, while scar formation with no skin cover was found in the control group at 4 weeks after implantation.（3） Findings from the interleukin 6 immumohistochemical staining showed that there was interleukin 6 positive expression in both two groups to different extents; at 4 weeks after implantation, the e

关 键 词：氧化锌 纳米粒 胶原 丝素蛋白 组织工程 纳米氧化锌 丝素胶原蛋白支架 创面修复 生物材料 炎症反应 皮肤创伤 

分 类 号：R318[医药卫生—生物医学工程]