阴极弧沉积掺硅二氧化锆膜对MG63细胞相关因子的影响  

Osteoclast activity of MG63 cells on silicon doped zirconia films prepared by cathodic arc deposition

作　　者：章筛林[1] 成翔宇[1] 石继祥[1] 周强[1] 石文俊[1] 刘孚瑛[1] 纪斌[1] Zhang Shai-lin;Cheng Xiang-yu;Shi Ji-xiang;Zhou Qiang;Shi Wen-jun;Liu Fu-ying;Ji Bin(Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, Chin)

机构地区：[1]上海中医药大学附属普陀医院骨科,上海市200062

出　　处：《中国组织工程研究》2018年第14期2227-2232,共6页Chinese Journal of Tissue Engineering Research

基　　金：上海市普陀区卫生系统自主创新项目(2013PTKW015);国家自然科学基金项目(81673782);上海市普陀区临床重点专科建设项目(2016PTZK04)~~

摘　　要：背景:采用各种表面改性方法提高医用钛及钛合金植入物的骨整合,是目前人工关节假体领域研究的重点之一。目的:在纯钛表面制备掺硅二氧化锆膜,研究其对成骨样MG63细胞相关因子的影响。方法:采用磁过滤真空阴极弧沉积技术在纯钛表面分别制备掺硅二氧化锆膜与二氧化锆膜。将成骨样MG63细胞分别接种于掺硅二氧化锆膜、二氧化锆膜及纯钛片表面,接种后第1,4,7,10天,以定量RT-PCR法检测细胞骨保护素和核因子κB受体活化因子配体基因表达情况,ELISA检测细胞分泌骨保护素和核因子κB受体活化因子配体蛋白量。结果与结论:(1)接种第1天,3组骨保护素基因及蛋白表达无差异;接种第4天,掺硅二氧化锆膜组、二氧化锆膜组骨保护素基因及蛋白表达高于纯钛组(P<0.05),掺硅二氧化锆膜组、二氧化锆膜组骨保护素基因及蛋白表达无差异;接种第7,10天,掺硅二氧化锆膜组骨保护素基因及蛋白表达高于二氧化锆膜组、纯钛组(P<0.05);(2)接种第1天,3组核因子κB受体活化因子配体基因及蛋白表达无差异;接种第4,7天,掺硅二氧化锆膜组核因子κB受体活化因子配体基因及蛋白表达低于二氧化锆膜组、纯钛组(P<0.05);接种第10天,掺硅二氧化锆膜组、二氧化锆膜组基因及蛋白表达低于纯钛组(P<0.05),掺硅二氧化锆膜组、二氧化锆膜组骨保护素基因及蛋白表达无差异;(3)结果表明,在纯钛表面阴极弧沉积掺硅二氧化锆膜,能够上调成骨样MG63细胞内骨保护素表达水平,同时下调核因子κB受体活化因子配体表达水平。BACKGROUND： Various surface modification techniques have been utilized as an attempt to improve the osteointegration of Ti-based implants, which is a hotspot research concerning the artificial joint prosthesis. OBJECTIVE： To evaluate the effect of silicon doped zirconia film on the titanium surface on relevant factors of osteoblast-like MG63 cells. METHODS： Silicon doped zirconia and zirconia films were respectively prepared on the titanium surface by cathodic arc deposition. Osteoblast-like MG63 cells were cultured on silicon doped zirconia film, zirconia film and pure titanium, respectively. After 1, 4, 7, 10 days of incubation, samples were collected for assay. Osteoprotegerin（OPG） and receptor activator of nuclear factor κB ligand（RANKL） at m RNA and protein levels were detected using real-time PCR and ELISA, respectively. RESULTS AND CONCLUSION：（1） At 1 day of inoculation, there was no significant difference in the OPG m RNA and protein expression among the three groups. At 4 days of inoculation, the expression of OPG m RNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group（P〈0.05）, but there was no significant difference between the former two groups. At 7 and 10 days of inoculation, the expression of OPG m RNA and protein in the silicon doped zirconia film growp was highest among the three groups（P〈0.05）.（2） At 1 day of inoculation, there was no significant difference in the RANKL m RNA and protein expression among the three groups. At 4 and 7 days of inoculation, the expression of RANKL m RNA and protein in the silicon doped zirconia film group was significantly lower than that in the other two groups（P〈0.05）. At 10 days of inoculation, the expression of OPG m RNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group（P〈0.05）, but there was no significant difference between the former two groups. T

关 键 词：钛 成骨细胞 骨保护素 核因子ΚB受体活化因子 组织工程 真空阴极弧沉积 二氧化锆 硅 生物材料 核因子ΚB受体活化因子配体 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]