Synthetic studies toward human interleukin-5  

作　　者：Jinrong Liu Suwei Dong 

机构地区：[1]State Key Laboratory of Natural and Biomimetic Drugs,Department of Chemical Biology,School of Pharmaceutical Sciences,Peking University

出　　处：《Chinese Chemical Letters》2018年第7期1131-1134,共4页中国化学快报（英文版）

基　　金：financial support from the National Recruitment Program of Global Youth Experts(1000 Plan);Peking University Health Science Center(No. BMU2017QQ008);the State Key Laboratory of Natural and Biomimetic Drugs

摘　　要：Interleukin 5(IL-5) is a human cytokine that regulates eosinophil growth and activation, and it plays essential roles in diseases associated with increased level of eosinophils such as asthma. Many studies have been conducted on this important glycoprotein, but all of them utilized recombinantly expressed samples. Here we describe our attempted chemical synthesis of IL-5, using protocols of Fmoc-SPPS and peptidyl hydrazide-based native chemical ligation, where the disulfide-reduced form of IL-5 is assembled from three peptide segments. Reconstitution of the protein under different folding conditions has also been investigated.Interleukin 5(IL-5) is a human cytokine that regulates eosinophil growth and activation, and it plays essential roles in diseases associated with increased level of eosinophils such as asthma. Many studies have been conducted on this important glycoprotein, but all of them utilized recombinantly expressed samples. Here we describe our attempted chemical synthesis of IL-5, using protocols of Fmoc-SPPS and peptidyl hydrazide-based native chemical ligation, where the disulfide-reduced form of IL-5 is assembled from three peptide segments. Reconstitution of the protein under different folding conditions has also been investigated.

关 键 词：Interleukin 5 Eosinophilic disorders ASTHMA Solid phase peptide synthesis Native chemical ligation Mirror-image phage display 

分 类 号：TQ460.6[化学工程—制药化工]