ISSR标记在生姜品种遗传多样性中的应用  

Application of ISSR in Genetic Diversity Analysis of Zingiber officinale Rosc.

作　　者：王向东[1] 王永存[1] 马艳芝[2] 宋玉双 付丽军[1] 李佳垚 WANG Xiangdong;WANG Yongcun;MA Yanzhi;SONG Yushuang;FU Lijun;LI Jiayao(Tangshan Academy of Agricultural Sciences, Hebei Tangshan 063001;Department of Life Sciences, Tangshan Normal University, Hebei Tangshan 063000;College of Life Sciences, Shandong Normal University, Jinan 250000 China)

机构地区：[1]唐山市农业科学研究院,河北唐山063001 [2]唐山师范学院生命科学系,河北唐山063000 [3]山东师范大学生命科学学院,济南250000

出　　处：《中国农业科技导报》2018年第7期42-47,共6页Journal of Agricultural Science and Technology

基　　金：河北省科技厅项目(16226344);唐山市农业科技推广项目(唐农科17005)资助

摘　　要：以8个姜品种的芽为试验材料,利用ISSR分子标记技术对它们进行遗传多样性分析。结果表明:筛选出的24条ISSR引物,共扩增出166个条带,其中55条为多态性条带,多态性比率为35.67%;8个姜品种的遗传距离在0.212 8~0.842 1之间,在λ=0.44处,可将供试的8品种分为3大类。供试8个姜品种多态性不高,5个自育品种和其他3个供试品种在遗传距离、亲缘关系、聚类结果各不相同,可进一步进行研究。Taking 8 sprouts of ginger varieties as materials,this paper analyzed and evaluated of the genetic diversity of 8 varieties of Zingiber officinale Rosc. by ISSR. The results showed that 24 ISSR primers were selected,and 166 bands were amplified with them,among which 55 bands were polymorphic,and their polymorphism percentage was35.67%. The genetic distance of 8 ginger varieties was between 0.212 8 ~ 0.842 1. At λ = 0.44,the materials could be divided into 3 categories. The genetic diversity of 8 germplasm resources was lower,5 self-varieties and 3 other varieties were different in genetic distance,genetic relationship,clustering,so they could be further studied.

关 键 词：姜 ISSR标记 遗传多样性 应用 

分 类 号：S632.5[农业科学—蔬菜学]