华北大黑鳃金龟成虫对三种植物的取食选择及趋性行为反应  

Feeding preference and taxis behavior of adult Holotrichia oblita( Coleoptera: Scarabaeidae) on three plants

作　　者：热孜宛古丽.阿卜杜克热木 曹雅忠[1] 张帅[1] 尹姣[1] 李雪[1] 李克斌[1] Rizwangul ABDUKERIM;CAO Ya-Zhong;ZHANG Shuai;YIN Jiao;LI Xue;LI Ke-Bin(State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2018年第5期585-595,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31371997;31572007);国家重点研发计划(2018YFD0201002)

摘　　要：【目的】本研究旨在明确华北大黑鳃金龟Holotrichia oblita成虫对3种植物的趋向选择性与取食偏好性的关系及其成因。【方法】利用Y型嗅觉仪和叶面积仪等分别测定华北大黑鳃金龟成虫对榆树Ulmus parvifolia、苘麻Abutilon theophrasti和蓖麻Ricinus communis 3种植物的趋向性和取食量,采用GC-MS分析鉴定这3种植物挥发物的成分,观测其对主要成分的嗅觉反应。【结果】华北大黑鳃金龟成虫对蓖麻的趋向性显著强于对苘麻和榆树的趋向性(P<0.05),对苘麻和榆树的趋向程度相当;但对榆树叶片的取食量最大(P<0.05),对苘麻和蓖麻叶片的取食量相近,雌虫与雄虫的取食量没有显著差异(P>0.05)。通过GC-MS分析鉴定发现,3种植物挥发物主要成分有明显差异。嗅觉反应结果显示,在10μg/μL浓度下,顺-3-己烯基异丁酸酯、邻苯二甲酸二丁酯、顺-3-己烯基乙酸酯、芳樟醇、苯酚、水杨酸甲酯和β-石竹烯等化合物对华北大黑鳃金龟的吸引力都显著强于对照(正己烷);但3-蒈烯、反-2-壬烯醛和α-水芹烯对华北大黑鳃金龟的吸引力弱于对照,并呈显著差异(P<0.05)。【结论】华北大黑鳃金龟成虫对榆树、苘麻和蓖麻的趋向选择性不等同于取食偏好性。【Aim 】 This study aims to clarify the relationship between feeding preference and taxis behavior of Holotrichia oblita adults on three plant species. 【Methods】The taxis and feeding amount of H. oblita adults to Chinese elm（ Ulmus parvifolia）,wild cotton（ Abutilon theophrasti）,and castor-oil plant（ Ricinus communis） were measured by the scanned leaf image device and Y-tube olfactometers.The volatiles from the three plants were collected by the dynamic headspace volatile collection method and identified by gas chromatography-mass spectrometer（ GC-MS）,and the active volatile compounds were screened by Y-tube olfactometer. 【Results】The taxis of H. oblita to R. communis was significantly higher than that to U. parvifolia and A. theophrasti（ P 〈0. 05）. There was no significant difference in taxis between to U. parvifolia and to A. theophrasti. However,H. oblita adults showed the highest foliarfeeding amount to U. parvifolia among these three plants,and displayed nearly equal taxis to A. theophrasti and R. communis. Moreover,no significant difference in foliar feeding amount was found between female and male adults of H. oblita（ P〉0. 05）. GC-MS analysis showed that plant volatile compounds in the three plants were largely different. In the olfactory response test with Y-tube olfactometer,（ Z）-3-hexenyl isobutyrate,dibutyl phthalate,（ Z）-3-hexenyl acetate,linalool,phenol,methyl salicylate,and β-caryophellen at the concentration of 10 μg/μL were found to be more attractive to H. oblita as compared with the control（ hexane）,while 3-carene,（ E）-2-nonenal and α-phellandrene were less attractive than hexane. 【Conclusion】The taxis of H. oblita to U. parvifolia,A. theophrasti and R. communis is not equivalent to its feeding preference.

关 键 词：华北大黑鳃金龟 寄主植物 取食选择性 植物挥发物 趋性反应 

分 类 号：Q968[生物学—昆虫学]