基于微卫星分子标记的中国瓜实蝇不同地理种群遗传分化分析  

Analysis of genetic differentiation of different geographical populations of the melon fly,Bactrocera cucuribitae( Diptera: Tephritidae ) in China based on microsatellite molecular markers

作　　者：张亚楠 牛黎明[1] 周世豪 龚治[1] 李磊[1] 张方平[1] 韩冬银[1] 符悦冠[1] ZHANG Ya-Nan;NIU Li-Ming;ZHOU Shi-Hao;GONG Zhi;LI Lei;ZHANG Fang-Ping;HAN Dong-Yin;FU Yue-Guan(Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海口571101 [2]海南大学热带农林学院,海口570228

出　　处：《昆虫学报》2018年第5期613-619,共7页Acta Entomologica Sinica

基　　金：国家科技支撑计划课题(2015BAD08B03);中国热带农业科学院基本科研业务费专项资金(1630042017014)

摘　　要：【目的】瓜实蝇Bactrocera cucuribitae是一种重要入侵害虫,寄主广,危害性大。本研究旨在揭示中国瓜实蝇种群分化和遗传变异情况。【方法】利用9个多态性微卫星位点作为分子标记对中国7个主要分布省份的21个地区共190头瓜实蝇样本进行遗传多样性分析。【结果】21个瓜实蝇地理种群多态位点百分率平均为97.08%,Shannon氏多样性指数I平均值为0.8841,种群间遗传分化系数FST=0.12806,说明已发生一定程度的遗传分化。UPGMA聚类分析显示,海南(不含三沙)、广东、广西、云南和海南三沙的瓜实蝇种群聚为一支,福建、江西和四川的瓜实蝇种群各自聚为一支。【结论】中国的瓜实蝇种群已发生一定程度的遗传分化,但积累的变异程度有限。【Aim】The melon fly,Bactrocera cucuribitae is an important invasive pest with wide host range,causing serious harm. This study aims to explore its population differentiation and genetic variation in China. 【Methods】The genetic diversity of a total of 190 individuals collected from 21 regions of 7 provinces in China was analyzed using nine polymorphic microsatellite loci as the molecular markers.【Results】For the 21 geographical populations of B. cucuribitae,the average percentage of polymorphic loci was 97. 08%,Shannon＇s diversity index（ I） was 0. 8841,and the genetic differentiation coefficient among populations（ FST） was 0. 12806,suggesting that genetic differentiation occurs among the 21 geographical populations. The UPGMA cluster analysis showed that the populations of Hainan（ excluding Sansha）,Guangdong,Guangxi,Yunnan and Sansha of Hainan were clustered into one clade,while the populations of Fujian,Jiangxi and Sichuan clustered into separate clades,respectively. 【Conclusion】A certain degree of genetic differentiation has occurred among the melon fly populations in China,but theaccumulated variability is limited.

关 键 词：瓜实蝇 地理种群 微卫星分子标记 遗传多样性 遗传分化 

分 类 号：Q963[生物学—昆虫学]