气虚血瘀证脑缺血再灌注病证结合大鼠模型的建立与评价  

Establishment and Evaluation on Combination of Disease and Syndrome Rat Model of Cerebral Ischemia/Reperfusion with Qi Deficiency Blood Stasis Syndrome

作　　者：谭辉[1] 尹婷婷[2] 王键[1] 何玲[1] 邓勇[1] 李净[1] 韩义皇 徐伟[1] TAN Hui;YIN Ting-ting;WANG Jian;HE Ling;DENG Yong;LI Jing;HAN Yi-huang;and XU Wei(College of TCM, Anhui Chinese Medical University, Hefei 230038;Department of Respiratory Medicine, Second Affiliated Hospital of Anhui Chinese Medical University, Hefei 230000;Graduate School, Beijing University of Chinese Medicine, Beijing 100029)

机构地区：[1]安徽中医药大学中医学院,合肥230038 [2]安徽中医药大学第二附属医院呼吸内科,合肥230000 [3]北京中医药大学研究生院,北京100029

出　　处：《中国中西医结合杂志》2018年第7期832-837,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金青年项目(No.81603523);安徽省高校自然科学研究重点项目(No.KJ2016A404);安徽中医药大学自然科学研究基金项目(No.2014zr001)

摘　　要：目的研究建立大脑中动脉闭塞缺血再灌注(MCAO-R)气虚血瘀证病证结合大鼠模型的制备方法和评价标准。方法雄性老龄SD大鼠40只,随机数字表法分成正常组、模型组、脑络欣通组、通心络组,每组10只。采用线栓法结合多因素老龄、饥饿、疲劳、高脂饮食、低氧等方法复合模拟制备气虚血瘀证MCAO-R模型,正常组和模型组予以0.9%氯化钠溶液灌胃,脑络欣通组和通心络组分别给予脑络欣通和通心络药液1 m L/(100 g·d)灌胃;采用激光多普勒仪、Morris水迷宫、TTC染色法、ELISA等方法检测各组大鼠神经功能缺损评分、气虚血瘀证评分、局部脑血流量(r CBF)、空间认知能力、脑梗死体积和凝血纤溶功能等指标变化。结果与正常组比较,模型组大鼠神经功能缺损评分、气虚血瘀证评分、脑梗死体积明显升高(P<0.01);与模型组比较,脑络欣通组和通心络组神经功能缺损评分、气虚血瘀证评分、脑梗死体积显著降低(P<0.01)。与正常组比较,模型大鼠空间认知能力、r CBF下降(P<0.01),与模型组比较,脑络欣通组和通心络组空间认知能力和r CBF升高(P<0.05)。与正常组比较,模型组凝血酶原时间(PT)、活化部位凝血活酶时间(APTT)和凝血酶时间(TT)均明显缩短,纤维蛋白原(FBG)含量升高(P<0.05,P<0.01),与模型组比较,脑络欣通组PT延长(P<0.01),FBG含量明显降低(P<0.01)。与正常组比较,模型组大鼠纤维蛋白原降解产物(FDP)和D-二聚体(D-D)水平升高(P<0.01),与模型组比较,脑络欣通组和通心络组大鼠FDP和D-D水平降低(P<0.01)。结论通过线栓法结合多因素复合模拟制备的大鼠模型出现了脑缺血的病理表现,同时具备中医气虚血瘀证的生物学特征,说明MCAO-R气虚血瘀证模型是较为理想的脑缺血病证结合模型。Objective To study preparation methods and evaluation criteria for combination of disease and syndrome rat model [middle cerebral artery occlusion-reperfusion（ MCAO-R） rats with qi deficiency blood stasis syndrome（ QDBSS） ]. Methods Totally 40 male old rats were divided into the normal group,the model group,the Naoluo Xingtong（ NLXT） group,and the Tongxinluo（ TXL） group,10 rats in each group. MCAO-R model was prepared by modified Longa EZ method. QDBSS was prepared by multiple factors complicated simulated ageing,hunger,fatigue,high fat diet,low oxygen,and so on. Rats in the normal group and the model group were administered with normal saline by gastrogavage. Thosein the NLXT group and the TXL group were administered with NLXT and TXL by gastrogavage（ 1 m L/100 g per day） respectively. Neural function defect score（ NFDS）,score for QDBSS,renal cortical blood flow（ r CBF）,spatial cognitive function,volume of cerebral infarct,and the function of blood coagulation and fibrinolysis were detected by laser Doppler scanning,Morris water maze,TTC staining,and ELISA. Results Compared with the normal group,NFDS,score for QDBSS,and volume of cerebral infarct obviously increased in the model group（P〈0. 01）. Compared with the model group,NFDS,score for QDBSS,and volume of cerebral infarct obviously decreased in the NLXT group and the TXL group（ P〈0. 01）.Compared with the normal group,spatial cognitive function and r CBF obviously decreased in the model group（P〈0. 01）.Compared with the model group,spatial cognitive function and r CBF obviously increased in the NLXT group and the TXL group（P〈0. 05）. Compared with the normal group,prothrombin time（ PT）,activated partial thromboplastin time（ APTT）,and thrombin time（ TT） were obviously shortened,the content of FBG increased in the model group（P〈0. 05,P〈0. 01）. Compared with the model group,PT was obviously prolonged,the content of FBG decreased in the NLXT group（P〈0. 01）. Compared with the normal group,t

关 键 词：脑缺血 气虚血瘀证 病证结合 局部脑血流量 凝血 益气活血法 

分 类 号：R285.5[医药卫生—中药学]