一株不依赖NAD生长的副鸡禽杆菌的分离与鉴定  

Isolation and identification of a NAD-independent Avibacterium paragallinarum

作　　者：马洪梅 牛家强 张海燕 刘洋洋 胡思顺[2,3] 许青荣[2] 李自力[2,3] 刘梅[2,3] 毕丁仁[2,3] MA Hong-mei;NIU Jla-qiang;ZHANG Hai-yan;LIU Yang-yang;HU Si-shun;XU Qing-rong;LI Zi-li;LIU Mei;BI Ding-ren(Provincial Key Laboratory of Tibet Plateau Animal Epidemic Disease Research, Zizang Agricultural and Animal Husbandry College, Department of Veterinary Medicane Nyingzhi, Tibet 860000, China;Key Laboratory of Development of Veterinary Diagnostic Products of Ministry Medicine, Huazhong Agricultural University, College of Veterinary Medicine, Wuhan 430070, China;Key Laboratory of Preventive Veterinary Medicine of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China)

机构地区：[1]西藏农牧学院动物科学学院/高原动物疫病研究自治区高校重点实验室,西藏林芝860000 [2]华中农业大学动物医学院/农业部兽用诊断制剂创制重点实验室,湖北武汉430070 [3]华中农业大学/湖北省预防兽医学重点实验室,湖北武汉430070

出　　处：《中国预防兽医学报》2018年第6期500-503,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：畜禽重大疫病防控与高效安全养殖综合技术研发重点专项(2016YFD05008004)

摘　　要：为研究湖北省内鸡传染性鼻炎病原菌的特性,本研究从湖北恩施某地鸡场疑似传染性鼻炎的病鸡样品中分离获得一株疑似副鸡禽杆菌(APG)的病原菌。通过细菌的分离培养、形态观察、生化试验、革兰氏染色、动物回归试验和PCR等方法对其进行鉴定。结果显示,分离的病原菌具有APG的形态特征和生化特性;16S rRNA序列比对分析与已报道的APG相应序列同源性高于98%;另外,利用特异PCR,分别检测到p250,pA14和pYMH5等3种质粒的特异基因片段;通过培养试验,该分离株能在无NAD的TSB(含鸡血清)中生长。这是国内首次发现无需NAD且同时存在3种质粒的APG。这一的发现警示国内的APG流行情况复杂,应引起畜牧兽医工作者的注意。Avibacterium paragallinarum is a causative agent of avian infectious coryza in chicken. To understand the characteristics of A.paragallinarum, a A.paragallinarum was isolated from chicken in Enshi, Hubei province, and identified by bacterial isolation and culture, morphological observation, biochemical tests, PCR and animal infection test. As results, the characteristics of the morphology, the biochemical tests and 16S rRNA sequence （more than 98% homology） of the isolate were identity to the A.paragallinarum reference strains. In addition, the isolate had the ability to grow in TSB medium containing chicken serum without NAD. Moreover, the 3 plasmids of p250, pAl4 and pYMH5 were detected in the isolate by PCR. This is the first report of NAD-independent and embodied the 3 plasmids of A.paragallinarum in China. Our results suggested that the A.paragallinarum infection was becoming more and more complex, which should be paid attention to by specialists.

关 键 词：副鸡禽杆菌 NAD 质粒 

分 类 号：S852.65[农业科学—基础兽医学]