TLR7激动剂作为狂犬病疫苗佐剂免疫效果的研究  

The effect of TLR7 agonist as adjuvant in rabies vaccine

作　　者：李慧 胡云龙[2] 朱士茂 梁琳琳 刘永娣 罗姗 郭采平 靳广毅[2] LI Hui;HU Yun-long;ZHU Shi-mao;LIANG Lin-lin;LILT Yong-di;LUO Shan;GUO Cai-ping;JING Guang-yi(Shenzhen Wciguang Biological Products Co., Ltd, Shenzhen 518107, China;Department of Pharmacy, School of Medicine, Shenzhen University, Shenzhen 518060, China)

机构地区：[1]深圳市卫光生物制品股份有限公司,广东深圳518107 [2]深圳大学医学院药学系,广东深圳518060

出　　处：《中国预防兽医学报》2018年第6期523-527,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：深圳市科技计划项目(JSGG20160331173111649)

摘　　要：为提高狂犬病疫苗对机体的保护效果,本研究将不同浓度的Toll样受体7小分子激动剂(T7)作为佐剂加入到rabies vaccine中记为rabies vaccine+T7,分别将其与小鼠脾淋巴细胞一起培养,检测不同T7浓度对其细胞因子IFN-γ和IL-12的刺激,筛选适合的T7浓度;制备该浓度的rabies vaccine+T7溶液,通过对小鼠细胞免疫能力、中和抗体水平以及保护效果等方面评价T7对rabies vaccine的免疫佐剂效应,并以含铝佐剂的rabies vaccine(rabies vaccine+Al)为对照。结果显示,5.6μmol/L^11.3μmol/L的T7在体外可诱导良好的IFN-γ和IL-12反应;在体内,rabies vaccine+T7免疫小鼠诱生的特异性IFN-γ斑点形成细胞(SFC)数均高于其它组,产生中和抗体水平的速度比rabies vaccine+Al组快,保护效果与rabies vaccine+Al组相当。本研究结果表明T7作为佐剂能在一定程度上提高rabies vaccine的效果,有望开发为新型人用rabies vaccine的佐剂。To improve the protective effect of rabies vaccine, different concentrations of toll-like receptors 7 agonist （T7） was added in the rabies vaccine （rabies vaccine ＋T7） as adjuvant, and cultivated with mice spleen lymphocytes. Then the cytokines IFN-3, and IL-12 were detected to evaluate the stimulating effect of T7. Mice were injected with the optimal concentration of rabies vaccine＋T7, and the cellular immunity, neutralizing antibody level and protective effect were detected to examine immune adjuvant effect of T7 for rabies vaccine, with aluminium adjuvant （rabies vaccine＋A1） as control. The results showed that T7 at the concentrations from 5.6 to l l.3p, mol/L was able to efficiently induce IFN-3, and 1L-12 reaction in vitro. In vivo, the rabies vaccine＋T7 group induced more specific IFN-γ spot formation cell （SFC） than that in other group, showed a faster speed in inducing neutralizing antibodies and similar protective effect as compared with rabies vaccine＋A1 group. These results demonstrate that T7 has the capability to improve the effect of rabies vaccine in certain extent and is a promising candidate to be used as a new adjuvant of rabies vaccine for human beings.

关 键 词：TLR7激动剂 狂犬病疫苗 佐剂 细胞因子 中和抗体 

分 类 号：S852.65[农业科学—基础兽医学]