抗细粒棘球绦虫疫苗候选抗原免疫犬IgG抗体水平监测及免疫组化定位研究  

IgG antibody level monitoring and immunohistochemical localization of dogs immunized with vaccine candidates against Echinococcus granulosus

作　　者：赵莉[1] 阿帕克孜.麦麦提 王俊伟[1] 张壮志[1] 官亚婷 王冰洁 刘志强[1] 穆尼拉.特列吾汗 刘明远[3] 吕凤军 丁伟强 ZHAO Li;APAKEZI· Maimaiti;WANG Jun-wei;ZHANG Zhuang-zhi;GUAN Ya-ting;WANG Bing-jie;LIU Zhi-qiang;MUNILA. Teliewuhan;LIU Ming-yuan;LV Feng-jun;D(Veterinary Research Institute, Xinjiang Academy of Animal Sciences, Urumqi 830011, China;College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China;Institute of Zoonosis, Jilin University, Changchun 130062, China;Animal Health inspection Institute of Midong District, Urumqi 831400, China)

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830011 [2]新疆农业大学动物医学院,新疆乌鲁木齐830052 [3]吉林大学人兽共患病研究所,吉林长春130062 [4]新疆乌鲁木齐市米东区动物卫生监督所,新疆乌鲁木齐831400

出　　处：《中国预防兽医学报》2018年第6期538-542,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家重点研发计划课题(2017YFD0501301);新疆自治区公益性基本科研业务费青年基金项目(KY2015013);NSFC-地区科学基金项目(31560692)

摘　　要：为探究细粒棘球绦虫(E.granulosus,Eg)成熟虫体(MAW)特异性蛋白(EgM)(EgM9、EgM123)的抗Eg感染保护效果,本研究利用Eg疫苗候选抗原EgM9和EgM123重组蛋白,分别3次免疫实验犬,利用ELISA法检测免疫犬血清中IgG、IFN-γ和IL-10的变化情况,采用免疫组化法检测免疫犬肠系膜淋巴结和小肠中的特异性IgG抗体。结果显示,在第3次免疫后一周即免疫组犬抗体水平均最高时对其进行原头蚴攻击,攻毒后免疫组的抗体水平随之下降,且持续至第3次免疫后19周,免疫组均与对照组呈显著性差异(p<0.05);第3次免疫后一周免疫组犬血清中IFN-γ和IL-10水平均呈升高趋势,且至第3次免疫后23周剖检,免疫组犬的肠系膜淋巴结和小肠组织经免疫组化法均检测出特异性IgG抗体。表明用EgM重组蛋白免疫犬,可诱导其产生IgG抗体而发挥抗Eg感染保护效果,为终末宿主犬包虫病的免疫机理探究和疫苗及相关检测试剂的研制奠定了基础。To evaluate the protection effect of the specific proteins of EgM9 and EgM123 from mature adult worms （MAW） of Echinococcus granulosus （EgM） against E.granulosus infection, the dogs were immunized with the vaccine candidates of recombinant protein EgM9 （rEgM9） and EgM123 （rEgM123）, three times for each protein, respectively. The changes of IgG, IFN-γ and IL-10 in the sera of the immunized dogs were detected by ELISA, and the specific IgG was localized in the mesenteric lymph nodes and small intestine by using immunohistochemical assay. The results showed that the antibody levels induced by rEgM9 or rEgM123 reached the highest through a week after the third injection （5 weeks）. The antibody levels of the immune groups dropped accordingly when dogs were challenged orally with protoscoleces. Until 23 weeks, the antibody levels were still significant differences between each of the immune groups and the control group （p〈0.05）. The levels of IFN-γ and IL-10 in the sera showed an elevated trend, and the specific IgG antibody was locally detected in the mesenteric lymph nodes and small intestine. Moreover, IgG antibody was still able to maintain in the sera, mesenteric lymph nodes and small intestine, respectively, until the experiment terminated necropsying at 27 weeks after immunization with rEgM9 or rEgM123. This study laid a foundation for the exploration of immune mechanism and the development of vaccines and related detection reagents.

关 键 词：细粒棘球绦虫 犬 EgM重组蛋白 抗体反应 免疫组织化学 

分 类 号：S855.9[农业科学—临床兽医学]