2016年～2017年山东地区商品肉鸡H9N2亚型禽流感病毒HA基因的分子特征和遗传进化分析  

Analysis on variation and molecular characteristics of hemagglutinin genes of H9N2 subtype avian influenza viruses isolated from Shandong in 2016-2017

作　　者：袁小远[1] 孟凯[1] 张玉霞[1] 徐怀英[1] 李莉[1] 艾武[1] 杨金兴[1] 亓丽红[1] 王友令[1] YUAN Xiao-yuan;MENG Kai;ZHANG Yu-xia;XU Huai-ying;LI Li;AI Wu;YANG Jin-xing;QI Li-hong;WANG You-ling(Institute of Poultry Research, Shandong Academy of Agricultural Sciences, Ji＇nan 250100, China)

机构地区：[1]山东省农业科学院家禽研究所,山东济南250100

出　　处：《中国预防兽医学报》2018年第6期543-545,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：山东省2017年度农业重大应用技术创新项目;山东省农业科学院农业科技创新工程(CXGC2016A10);山东省农业科学院院青年基金项目(2014QNM15;2016YQN56)

摘　　要：为了解山东地区商品肉鸡H9N2亚型禽流感病毒(AIV)分离株的遗传变异情况,本研究对13株从不同肉鸡场分离的H9N2 AIV的HA基因进行PCR扩增、克隆和测序,并对获得的HA基因序列进行同源性和遗传进化分析。结果显示:13个分离株的HA裂解位点均为RSSR↓GLF,符合低致病性AIV的分子特征;8株分离株有8处糖基化位点,其余5株存在个别位点缺失情况;受体结合位点除198位有变异外,其它位点均保守;234位氨基酸均为L,因此具有与哺乳动物唾液酸α,2-6受体结合的特征。所有分离株HA基因核苷酸同源性为94.1%~99.8%,氨基酸同源性为94.3%~100%。HA基因进化树显示,所有分离株均属于欧亚分支中的A/Chicken/Beijing/1/1994(A/Duck/Hong Kong/Y280/97)亚群,也同属于近几年在中国鸡群中流行的以A/chicken/Zhejiang/HJ/2007为代表的G57分支。以上研究为H9N2亚型AIV的防控和疫苗研制提供了科学参考。To understand the genetic variation of H9N2 subtype avian influenza virus （AIV） in Shandong province, HA genes of 13 isolates from different broiler farms were amplified, cloned and sequenced. The homology and genetic evolution of these HA genes were analyzed and showed that these isolates had a common motif RSSR$ GLF at the cleavage site which indicated that they were lentogenic. The deletion of glycosylation sites existed in five isolates, the other had eight glycosylation sites. In addition to the 198th site, other receptor binding sites were conservative. The 234 amino acids were L, which had the characteristics of binding to mammalian sialic acid tx,2-6 receptor. The nucleotide homologies of HA genes of the 13 isolates ranged from 94.1% to 99.8%, and amino acid homologies were from 94.3% to 100%. Phylogenetic tree of HA genes analysis showed that all isolates belonged to the Eurasian branch of the A/Chicken/Beijing/1/1994 （A/Duck/HongKong/Y280/97） subgroup, also belonged to the branch of G57, which have been prevalence in China among chick flocks in recent years represented by A/chicken/Zhejiang/HJ/ 2007. These data provided a reference for the prevention and control of H9N2 subtype AIVs and the vaccine development.

关 键 词：禽流感病毒 H9N2亚型 HA基因 序列分析 遗传进化 

分 类 号：S852.65[农业科学—基础兽医学]