DEHP诱导自噬抑制原始卵泡发育  

Di(2-ethylhexyl) Phthalate Inhibited Primordial Follicle by Inducing Autophagy in Mice Ovary

作　　者：张妍 穆欣艺[1] 耿艳清 何俊琳[1] 刘学庆[1] 陈雪梅[1] 丁裕斌[1] 王应雄[1] 高茹菲[1] Zhang Yah;Mu Xinyi;Geng Yanqing;He Junlin;Liu Xueqing;Chen Xuemei;Ding Yubin;Wang Yingxiong;Gao Rufei(Laboratory of the Reproductive Biology, School of Public Health and Management, Chongqing Medical University~Joint lnternational Research Laboratory of Reproduction ＆ Development, Chongqing 400016, China)

机构地区：[1]重庆医科大学公共卫生与管理学院生殖生物学实验室/教育部生殖与发育国际合作联合实验室,重庆400016

出　　处：《中国细胞生物学学报》2018年第5期725-730,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31571554);重庆市教委研究生科研创新项目(批准号:CYS16141)资助的课题~~

摘　　要：该文探讨了自噬在邻苯二甲酸二(2-乙基己)酯[Di(2-ethylhexyl)phthalate,DEHP]所引起的围产期卵巢原始卵泡发育异常这一过程中的作用。将BALB/C鼠随机分为对照组、DEHP组、DEHP与3-MA联合处理组、DEHP与Rapa联合处理组。新生鼠连续腹腔注射5天,孕16.5天(days post coitum,dpc)胎鼠卵巢体外培养6天后收集卵巢组织。H&E染色发现,与对照组相比,DEHP组原始卵泡比例明显减少,合胞体卵母细胞增多。TME检测发现,DEHP组存在明显的自噬小体。Western blot发现,DEHP组自噬标志分子LC3和Beclin1蛋白质水平表达显著升高,提示自噬增强。进一步干预自噬发现,DEHP与自噬抑制剂3-MA联合处理后,原始卵泡比例较DEHP单独处理组得到一定程度的恢复。而DEHP与自噬诱导剂Rapa联合处理后,原始卵泡比例进一步减少。该研究结果提示,围产期DEHP暴露可通过诱导自噬抑制原始卵泡发育。To investigate the effect of autophagy on Di（2-ethylhexyl） phthalate（DEHP） inhibited primordial folliculogenesis in perinatal mice ovaries, the BALB/C mice were randomly divided into control group, DEHP group, DEHP and 3-MA combinative treatment group, and DEHP and Rapa combinative treatment group. The neonatal female mice were injected once a day from birth to 5 days postpartum（5 dpp）, and 16.5 days post coitum（dpc） fetal mice ovaries were cultured for 6 days. The results showed that the percent of primordial follicles decreased significantly and the percent of cyst-oocytes increased obviously in DEHP group, compared with control group. Furthermore, DEHP activated autophagy in the ovary, with increased autophagy-related gene expressions and recognizable autophagosomes, however, inhibition of autophagy by 3-MA attenuated the adverse impact of DEHP on primordial folliculogenesis, moreover, induction of autophagy by Rapa strengthened the adverse impaction. This study demonstrated that perinatal exposure to DEHP induces autophagy to inhibit primordial folliculogenesis in mice ovary.

关 键 词：DEHP 原始卵泡 自噬 卵巢 

分 类 号：R714.7[医药卫生—妇产科学]