低温对桂花‘状元红’叶肉细胞超微结构的影响  

Effects of Low Temperature on Mesophyll Cell Ultrastructure of Osmanthus fragrans ‘Zhangyuan Hong'

作　　者：尤扬 王贤荣 张晓云 You Yang;Wang Xianrong;Zhang Xiaoyun(College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China;School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China)

机构地区：[1]南京林业大学生物与环境学院,南京210037 [2]河南科技学院园艺园林学院,新乡453003

出　　处：《中国细胞生物学学报》2018年第5期752-758,共7页Chinese Journal of Cell Biology

基　　金：河南省科技计划项目(批准号:162102110155);河南省高等学校重点科研项目(批准号:15A220002);河南教育厅科学技术研究重点项目(批准号:13A180307);新乡市科技创新发展专项项目(批准号:CXGG16029)资助的课题~~

摘　　要：为探讨北引桂花(Osmanthus fragrans)在低温胁迫下叶肉细胞超微结构的变化,揭示桂花于低温胁迫下细胞结构变化规律,该研究以3年生桂花品种‘状元红’(O.fragrans‘Zhuangyuan Hong’)为试材,分别于一系列低温下处理,经制样切片后,用透射电子显微镜观察叶肉细胞超微结构的变化。结果表明:常温(20~25°C)处理时,各细胞器超微结构正常;5°C低温处理时,叶绿体有轻微膨大现象,线粒体结构正常;0°C处理时叶绿体内嗜锇体增多,叶绿体肿胀加剧,线粒体数量增加,淀粉粒出现亮暗相间的轮纹;–10°C处理时,细胞器降解。在同一低温胁迫下不同细胞的叶绿体敏感程度不同,这为遭受低温后植株的恢复生长提供了细胞学基础。叶肉细胞中叶绿体、线粒体、细胞核的稳定性可作为桂花对低温响应的重要参考指标。To investigate the ultrastructural change in the mesophyll cells of Osmanthus fragrans which was introduced from southern to northen China and reveal the regulation of cell construction changes under the low temperature stress conditions, the investigation used 3-year-old trees of O.fragrans ‘Zhuangyuan Hong＇ as material and put them a series of low temperature. After sampling and chiping, using transmission electron microscope to observe the change of mesophyll cells ultrastructural. The ultrastructure of every organelle was normal at the normal outside temperature（20-25 °C）. At 5 °C, chloroplasts underwent a slight swelling, while mitochondrial structures were normal. At 0 °C, the osmiophilic granules in chloroplasts increased, chloroplast swelled, and the total number of mitochondria increased, while the starches appeared annulated between bright and dark interphase. At-10 °C, the organelles collapsed and degraded. Therefore, the chloroplasts of different cells exposed to low temperature showed different degrees of sensitivity to low-temperature. These results provided cytological basis for the recovery and growth of the plants. The stabilization of chloroplasts, mitochondria and nuclei in the mesophyll cells can be used as important reference indices for the response of O. fragrans to low temperature.

关 键 词：桂花 '状元红’ 超微结构 低温胁迫 

分 类 号：S685.13[农业科学—观赏园艺]