姜黄素预处理对干热环境大鼠骨骼肌组织学及超微结构变化的影响  

The Changes of Histopathology and Ultramicrostructure of Skeletal Muscle in Curcumin Pretreatment Rats Under Dry Heat Environment

作　　者：张东辉[1] 薛晓玉 谢志 唐军[1] 李建瑛[1] 许琴[1] 是文辉[1] 李佳佳[1] 董翔[1] 马娜[1] 宋来阳[1] 刘江伟[1] ZHANG Donghui;XUE Xiaoyu;XIE Zhi;TANG Jun;LI Jianying;XU Qin;SHI Weihui;LI Jiajia;DONG Xiang;MA Na;SONG Laiyang;LIU Jiangwei(Key Laboratory of Special Environmental Medicines of Xinjiang, General Hospital of Xinjiaag Military Command, Urumqi 830000, Chin)

机构地区：[1]新疆军区总医院新疆特殊环境医学重点实验室,乌鲁木齐830000

出　　处：《实验动物科学》2018年第3期1-6,共6页Laboratory Animal Science

基　　金：军队临床高新技术重大项目(No.2010gxjs016)

摘　　要：目的探讨姜黄素预处理对干热环境暴露大鼠骨骼肌的影响。方法选取6~8周龄SPF级雄性SD大鼠200只,每组40只,随机分组(盐水组、溶剂组、姜黄素低剂量预处理组、姜黄素中剂量预处理组、姜黄素高剂量预处理组),各组连续7 d等体积灌胃,第8天置于西北特殊环境人工实验舱,设定沙漠干热环境气候模式[(温度(41±0.5)℃,湿度(10±1)%)],分别在0 min、50 min、100 min、150 min时间点,各组随机取10只出舱麻醉,采集下腔静脉血液用于测定肌红蛋白含量;取部分股二头肌于甲醛溶液中固定用于光镜下观察,随机选取5个视野对白肌进行灰度测量,于50 min、150 min从盐水组、姜黄素高浓度组大鼠股二头肌中取1 mm×1mm×1 mm组织块置于锇酸中固定用于透射电镜观察。结果 1.光镜下观察结果显示:盐水组、姜黄素高浓度组在干热环境中随时间延长同根肌纤维部分区域颜色变淡,变淡区域逐渐增多,盐水组变淡区域逐渐变为白色,姜黄素高浓度组变淡区域呈淡红色;并且随时间延长颜色变淡的肌纤维数量逐渐增多,尤其盐水组增多明显;2.透射电镜观察表明干热环境盐水组以线粒体减少为主要组织学改变;姜黄素高浓度组与盐水组相比线粒体数量减少的较少,姜黄素高浓度组可见线粒体堆集的增生迹象;3.干热环境中各组血液中肌红蛋白含量无显著变化(P>0.05);4.灰度扫描结果提示:盐水组和溶剂组,从0 min到150 min,灰度值逐渐升高,150 min明显高于0 min组(P<0.05);在各个时间点(0 min除外),随着姜黄素浓度增加,灰度值均呈下降趋势,在50 min组差异无显著性(P>0.05);在100 min和150 min时,姜黄素中、高浓度组灰度值较溶剂组和盐水组下降明显,具有显著性差异(P<0.05)。结论姜黄素预处理能够减少干热环境大鼠骨骼肌从红肌转化为白肌的数量,对骨骼肌线粒体的减少具有一定的抑制作用,提示姜黄素预处理可能在一定Objective To observe the changes of histopathology and ultramicrostrcture of skeletal muscle of curcumin pretreatment rats in the dry-heat environment. Method 200 male rats were randomly devided into 5 groups（n = 40） ： Saline group,solvent group,low dose curcumin pretreatment group,medium dose curcumin pretreatment group,high dose curcumin pretreatment group. All groups were given a isometric gavage for 7 days. At the 8 thday,all groups were transferred to the Simulated Climate Cabin for Special Enviroment of Northwest of China with the setting environment： temperature（41 ± 0. 5） ℃,relative humidity（10 ± 1） %. At the time of 0 th min,50 th min,100 th min,150 th min,10 rats per group were gotten out from the cabin and anesthettized for collecting inferior vena cava blood for the determination of myoglobin, musculi biceps femoris were harvested and fixed in formalin for HE staining and pathological observation by optical microscope. Selected randomly from 5 field of view to measure gray scale of muscle under 10 × 10 microscope len. At 50 th min and 150 th min,the 1 mm × 1 mm ×1 mm tissue of rat musculi biceps femoris of saline group and high dose curcumin pretreatment group were fixed in osmium acid and used for transmission electron microscope observation,respectively. Result 1. The observation result under optical microscope showed： The color of part of muscle fiber of saline group and high dose curcumin pretreatment group fade and pale area gradually increased with the prolonging time in dry heat environment. Saline group fading region gradually fades to white,however,high dose curcumin pretreatment group pales reddish color;In addition,the number of fading muscle fibers gradually increased with time,especially in the saline group. 2.Transmission electron microscopy showed that the mitochondria decreased as the main histological change in the dry heat environment,the change in the high dose curcumin pretreatment group was less than that in the saline group.The mitochondrial heap can be s

关 键 词：姜黄素 干热环境 大鼠 骨骼肌 肌红蛋白 线粒体 

分 类 号：Q494[生物学—生理学]