BABL/c突变卷毛小鼠免疫学特性指标的分析  

Analysis of Immunological Characteristics of BABL/c Mutant Haired Mice

作　　者：李晓娟[1] 张巧云 汤紫荣[3] 冯帆 孙慧伟 李润 李瑞生[1] LI Xiaojuan;ZHANG Qiaoyun;TANG Zirong;FENG Fan;SUN Huiwei;LI Run;LI Ruisheng(Research Center for Clinical Medicine, 302 Hospital of PLA, Beijing 100039, China;Test Department of Chinese Medicine Hospital in Penglai of Shandong Province, Penglai 265600, China;Infection control department,302 Hospital of PLA, Beijing 100039, China)

机构地区：[1]解放军第302医院临床研究管理中心,北京100039 [2]山东省蓬莱市中医医院检验科,蓬莱265600 [3]解放军第302医院疾病预防控制科,北京100039

出　　处：《实验动物科学》2018年第3期25-28,共4页Laboratory Animal Science

基　　金：军队实验动物专项基金面上项目(No.SYDW(2016)-005)

摘　　要：目的探讨BALB/c突变卷毛小鼠与BALB/c小鼠之间的免疫学指标是否存在差异。方法选择6周龄的BALB/c突变卷毛小鼠和BALB/c小鼠各20只(雌雄各半),分别使用全自动血细胞分析仪检测6项血液免疫细胞指标;使用全自动生化仪检测4项抗体和补体指标;ELISA法检测血清细胞因子TNF-α、IL-2、IL-4水平;并对两组结果进行对比分析。结果两组小鼠NE、NE#和MO的雌性及组间结果相比较具有显著性差异(P<0.05,P<0.01),两组小鼠LY的雌性结果相比较具有显著性差异(P<0.05);两组小鼠IgM的性别及组间结果相比具有显著性差异(P<0.05,P<0.01),两组小鼠的IgG和C3的雄性及组间结果相比较具有显著性差异(P<0.01),两组小鼠IL-4的雌性及组间结果相比较具有显著性差异(P<0.05)。结论 BALB/c突变卷毛小鼠和BALB/c小鼠的血液免疫学指标存在差异,提示突变基因可能对小鼠的免疫系统产生了一定的影响。Objective To investigate whether there is a mutation difference between the immunological indexes of BALB/c mutant mice and the normal BALB/c mice. Method Six weeks old BALB/c mutant curly and normal BALB/c mice each 20（male and female） were selected respectively. Then 6 blood routine indexes were detected using automatic blood cell analyzer; 4 antibody and complement indexes were detected using automatic biochemical analyzer; and the ELISA was used to detect serum cytokines TNF-α、IL-2、IL-4; the result of the two groups were compared. Result The female and group result of NE、NE#、MO、and IL-4 between BALB/c mutant curly mice and normal mice have significant differences（P〈0. 05,P〈0. 01）; the male and group result of IgG and C3 between BALB/c mutant curly mice and normal mice have significant differences（P〈0. 01）; the sex and group result of IgM between BALB/c mutant curly mice and normal mice have significant differences（P〈0. 05,P〈0. 01）; the female result of LY between BALB/c mutant curly mice and normal mice have significant differences（P〈0. 05）.Conclusion There are differences in immunological parameters between BALB/c mutant curly mice and normal BALB/c mice,suggesting that the mutant gene may have a certain effect on the immune system of mice.

关 键 词：BALB/C小鼠 突变卷毛小鼠 血液免疫学指标 

分 类 号：R392[医药卫生—免疫学]