复方安神定志灵方对ADHD模型大鼠Akt、GSK-3β、β-catenin蛋白及mRNA的影响  

Effects of Anshen Dingzhi Ling Decoction on protein and mRNA of Akt, GSK-3β, β-catenin in ADHD model rats

作　　者：孙继超[1] 张碧霞[2] 韩新民[1] 顾国祥 尹东奇[1] SUN Ji-chao;ZHANG Bi-xia;HAN Xin-min;GU Guo-xiang;YIN Dong-qi(Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Yancheng Maternity and Child Health Care Hospital, Yancheng, 224001, China;Yancheng Hospital of Traditional Chinese Medicine, Yancheng, 224001, China)

机构地区：[1]南京中医药大学儿科研究所,南京210023 [2]盐城市妇幼保健院,江苏盐城224001 [3]盐城市中医院,江苏盐城224001

出　　处：《中华中医药杂志》2018年第7期3031-3034,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81273801)~~

摘　　要：目的:探讨安神定志灵方对自发性高血压大鼠(SHR)前额叶、纹状体及海马中Akt/GSK-3β/β-catenin信号通路的影响。方法:将50只SHR大鼠随机分为模型组、利他林组(2mg/kg)、安神定志灵低、中、高剂量组(6.7、13.4、26.7g/kg),每组10只,另将10只Wistar京都大鼠设为正常组。每日灌胃2次,治疗4周后取大鼠脑组织。分别用蛋白质印迹法(Western Blot)、免疫组织化学法及实时定量荧光PCR检测各组大鼠前额叶、纹状体、海马组织中Akt、GSK-3β和β-catenin的表达水平。结果:与正常组比较,模型组大鼠前额叶、纹状体中β-catenin、Akt的蛋白及mRNA表达降低,GSK-3β相关表达增加;海马区β-catenin、Akt的OD值显著性降低(P<0.05),GSK-3β的OD值显著性升高(P<0.05)。治疗后与模型组比较,利他林及安神定志灵组能够增加大鼠前额叶、纹状体中β-catenin、Akt的蛋白及mRNA表达,降低GSK-3β的相关表达;增加大鼠海马区β-catenin、Akt的OD值,降低GSK-3β的OD值。结论:安神定志灵可以影响Akt、GSK-3β、β-catenin表达水平,其药效可能与抑制细胞凋亡、保护神经元存在关联。Objective： To explore the effects of Anshen Dingzhi Ling（ADL） Decoction on Akt/GSK-3β/β-catenin signaling pathway of the prefrontal, striatum, and hippocampus in spontaneously hypertensive rats（SHR）. Methods： Fi Fty SHR were randomly divided into model group, ritalin group（2 mg/kg）, Anshen Dingzhi Ling Decoction low, medium, high dose group（6.7, 13.4, 26.7 g/kg respectively）, with ten rats in each group, and ten Wistar-Kyoto rats were set as the normal group. The rats were treated 2 times a day for four weeks, and then they were sacrificed to take brain tissues. Western Blot, immunohistochemistry and real-time PCR were used to detect the expression levels of Akt, GSK-3β,β-catenin in SHR striatum, prefrontal cortex and hippocampus. Results： Compared with the normal group, the protein and mRNA expression of dex and Akt in the frontal lobe and striatum of the model group were decreased, and GSK-3 related expression was increased. In the hippocampal area, the OD value of caec-catenin and Akt was significantly decreased（P〈0.05）, while the OD value of GSK-3 was significantly increased（P〈0.05）. After treatment, compared with the model group, ritalin and anshen dingling group can increase the protein and mRNA expression of-catenin and Akt in the frontal lobe and striatum of rats, and reduce the related expression of GSK-3. Increase the OD value of the hippocampal region of the rat and decrease the OD value of GSK-3. Conclusion： Anshen Dingzhi Ling Decoction can influence the expression levels of Akt, GSK-3β and β-catenin, and its efficacy may be associated with inhibiting apoptosis and protecting neurons.

关 键 词：安神定志灵 注意缺陷多动障碍 蛋白激酶B 糖原合成酶激酶-3Β Β-连环蛋白 自发性高血压大鼠 

分 类 号：R285.5[医药卫生—中药学]