神经调节蛋白-1对大鼠心肌缺血再灌注的影响  

The effect of neuregulin - 1 on myocardial ischemia reperfusion injury in rats

作　　者：吴振华[1] 郭志刚[1] 齐玉娟[1] 李培军[1] Wu Zhenhua;Guo Zhigang;Qi Yujuan;Li Peijun(Department of Cardiac Surgery, Tianjin Thoracic Hospital, Tianjin 300222, Chin)

机构地区：[1]天津市胸科医院心脏外科,300222

出　　处：《中华实验外科杂志》2018年第7期1280-1282,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨神经调节蛋白-1（NGR-1）对大鼠心肌缺血再灌注的影响及其机制。方法构建大鼠心肌缺血再灌注模型，随机分为模型组和NGR-1组。同时设置假手术组，假手术组左冠状动脉前降支不结扎。NRG-1组大鼠在造模前静脉注射NRG-1。取大鼠心脏，氯化三苯基四氮唑（TTC）法检测心肌梗死面积。分离大鼠心肌细胞，流式细胞仪检测细胞凋亡。检测心肌组织中B细胞淋巴瘤／白血病-2（bcl-2）、bcl-2相关X蛋白（bax）、信号转导和转录因子3（STAT3）、磷酸化的STAT3（p-STAT3）蛋白水平、活性氧（ROS）水平、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。结果模型组大鼠心肌梗死面积高于假手术组（P=0．000），NRG-1组大鼠心肌梗死面积低于模型组（P=0．000）。模型组大鼠心肌细胞凋亡率明显多于假手术组（P=0．000），NRG-1组大鼠心肌细胞凋亡率明显低于模型组（P=0．000）。模型组大鼠心肌组织中bcl-2、p-STAT3水平明显低于假手术组（P=0．000），NRG-1组大鼠心肌组织中bcl-2、p-STAT3水平高于模型组（P=0．000）。模型组大鼠心肌组织中bax水平高于假手术组（P=0．000），NRG-1组大鼠心肌组织中bax水平低于模型组（P=0．000）。模型组大鼠ROS水平、MDA含量明显高于假手术组（P=0．000）。NRG-1组大鼠ROS水平、MDA含量明显低于模型组（P=0．000）。模型组大鼠中SOD活性明显低于假手术组（P=0．000）。NRG-1组大鼠SOD活性明显高于模型组（P=0．000）。结论NRG-1能够有效改善心肌缺血再灌注损伤，作用机制与调控心肌细胞凋亡、自由基水平和STAT3信号通路有关。Objective To investigate the effect and mechanism of neuregulin - 1 （ NRG - 1 ） on myocardial ischemia reperfusion injury in rats. Methods The model of myocardial ischemia and reperfusion in rats were randomly divided into the model group and the NGR - 1 group. At the same time, set up the sham operation group, and the left anterior descending artery of the sham operation group was not ligation. NRG - 1 group rats were injected with NRG - 1 before modeling. Three phenyl tetrazolium chloride （TFC） method was used to detect the myocardial infarct size in rat heart. Isolated rat myocardial cells, flow cytometry was used to detect cell apoptosis. Detection of B cell lymphoma/leukemia 2 （ bcl - 2 ） , bcl-2 related X protein （bax）, signal transduction and transcription factor 3 （STAT3）, phosphorylated STAT3 （ p - STAT3 ） protein and reactive oxygen species （ROS） level, SOD activity and malondialdehyde （MDA） content in cardiac tissue. Results The myocardial infarction area of model group was higher than that of sham operation group （ P = 0. 000）, and the infarct size of NRG - 1 group was lower than that of model group （ P = 0. 000）. In model group, myocardial apoptosis was significantly more than that in sham operation group （P = 0. 000）, the rate of myocardial apoptosis in NRG - 1 group was significantly lower than that in model group （P = 0. 000）. The myocardial tissue of bcl - 2 and p - STAT3 levels in the model rats were significantly lower than the sham operation group （ P = 0. 000） , bcl - 2 and p - STAT3 level of myocardial tissue in NRG - 1 rats was higher than in the model group （ P = 0. 000）. The level of bax in myocardial tissue of rats in the model group was higher than in the sham operation group （P = 0. 000） , the bax level of myocardial tissue in NRG - 1 rats was lower than the model group （P = 0. 000）. The levels of ROS and MDA in the model group were significantly higher than those in the sham operation group （ P = 0. 000）. The levels o

关 键 词：心肌缺血再灌注 神经调节蛋白-1 凋亡 氧自由基 

分 类 号：R54[医药卫生—心血管疾病]