武当山地区3种娑罗子中β-七叶皂苷A、B提取工艺的比较  

Comparison of Different Extraction Methods of β-aescin A,B from Three Different Aesculi Semens in Wudang Area

作　　者：曹源源 张晨宁 朱美玲[2] 杨光义 CAO Yuan-yuan;ZHANG Chen-ning;ZHU Mei-ling;YANG Guang-yi(Department of Pharmacy, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000;Baoan Hospital of Traditional Chinese Medicine, Shenzhen , Gnangdong 518001, China)

机构地区：[1]十堰市太和医院.湖北医药学院附属医院,湖北十堰442000 [2]深圳市宝安区中医院,广东深圳518101

出　　处：《湖北医药学院学报》2018年第2期161-163,共3页Journal of Hubei University of Medicine

基　　金：湖北省卫计委科研项目(WJ2017F079);十堰市科学技术研究与开发项目计划(16Y20);湖北医药学院附属太和医院基金项目(2016JJXM049)

摘　　要：目的:本文旨在探索四种不同提取方法对武当山地区3种娑罗子(野生、半野生、人工种植)中的β-七叶皂苷A、B的提取效果,并同时分析两种化合物的含量。方法:分别采用醇提法、回流提取法、超声提取法、索氏提取法等方法提取娑罗子中的β-七叶皂苷A、B,采用高效液相色谱法测定各种提取方法所得β-七叶皂苷A、B的含量。色谱条件:Sun Fire TM LC-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.2%磷酸水溶液(37∶63);检测波长为UV 220 nm,流速为1.0 mL/min,柱温为35℃。结果:不同提取方法对娑罗子中β-七叶皂苷的提取率影响较大,其中索氏提取法提取效率最高,而超声提取法提取效率最低。结论:采用不同的提取方法对含量测定的结果影响较大,索氏提取法为本次试验的最佳提取方法。Objective To explore four different extraction methods of β-aescin A,B from three different varieties of Semen Aesculi in Wudang Mountain area（ wild,semi-wild,artificial planting） and determine the content of β-aescin A,B,and to determine the contents of β-aescin A and β-aescin B,simultaneously. Methods The alcohol extraction method,reflux extraction method,ultrasonic extraction method and soxhlet extraction method were used respectively. The content of β-aescin was determinated by HPLC method. Chromatographic conditions： a Sun Fire TM LC-C18 column（ 4.6 mm×250 mm,5μm） was used. The solution of acetonitrile-0.2% phosphoric acid solution（ 37 ∶ 63） was used as the mobile phase at a flow rate of 1.0 mL/min and the detection wavelength was 220 nm. Separation was performed at 35 ℃. Results The efficiency of different extraction methods on the extraction yield of β-aescin were different. The efficiency of soxhlet extraction method was high and the ultrasonic extraction method was low. Conclusion The extraction method plays an important role in the extraction of β-aescin from Semen Aesculi,in which the soxhlet extraction method was the best.

关 键 词：娑罗子 提取工艺 HPLC法 七叶皂苷A、B 

分 类 号：R284.2[医药卫生—中药学]