Minifish dazl和dnd基因的克隆及表达分析  

Cloning and Expression Analysis of dazl and dnd Genes in Minifish

作　　者：陈克让 丁天宜 李名友 Chen Kerang;Ding Tianyi;Li Mingyou(Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Ministry of Agriculture, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306)

机构地区：[1]上海海洋大学水产与生命学院农业部淡水水产种质资源重点实验室

出　　处：《基因组学与应用生物学》2018年第7期2795-2803,共9页Genomics and Applied Biology

基　　金：国家自然科学基金(31372520;31672700);上海科学技术委员会浦江人才计划(15PJ1403100);上海市教育委员会曙光计划共同资助

摘　　要：Minifish（Paedocypris progenetica）是目前世界上发现的个体最小的鱼,是探索生物进化及多样性的优良脊椎动物。当前,受人类活动影响,其生存空间日渐压缩,种群数量快速减少,研究该物种的生殖发育对维持及扩大其种群数量具有重要意义。本研究首次克隆了minifish dazl（Pp-dazl）和dnd（Pp-dnd）基因,Pp-dazl c DNA长为678 bp核酸,编码218个氨基酸;Pp-dnd c DNA长为1 071 bp核酸,编码352个氨基酸。氨基酸比对分析显示,Pp-Dazl和Pp-Dnd蛋白含有保守的RNA结合结构域。系统进化树分析表明,Pp-dazl和Pp-dnd与鲤科鱼类的同源蛋白亲缘关系最近。RT-PCR表明Pp-dazl和Pp-dnd RNA具有组织表达特异性,仅在成体组织中的性腺如卵巢中表达。本研究预示了Pp-dazl和Pp-dnd RNA在minifish性腺及生殖发育中具有重要作用,为minifish的生殖发育研究提供了基础信息。Minifish （Paedocypris progenetica） is the smallest fish in the world and one of the most perfect vertebrate species to study biological evolution and diversity. In recent years, the living spaces of minifish have been shrinked and the population has been rapidly reduced due to the activities of human beings. It is of great significance to study the reproductive development of this species to maintain and expand its population. This research cloned dazl （Pp-dazl） and dnd （Pp-dnd） genes in minifish for the first time. The cDNA ofPp-dazl contained 678 bp nucleotides, encoding 218 aa （amino acid, aa）. The cDNA of Pp-dnd contained 1 071 bp nucleotides, encoding 352 aa. Amino acid alignment revealed that both Pp-Dazl and Pp-Dnd had a conserved RNA binding domain. Phylogenetic tree analysis showed that Pp-Dazl and Pp-Dnd of minifish were the most related to the homologous proteins of Cyprinidae. RT-PCR indicated that Pp-dazl and Pp-dnd RNA were expressed specifically in tissues and only expressed in gonads such as ovaries in adult tissues. This study predicted that Pp-dazl and Pp-dnd may play an important role in the gonad and reproductive development, and might provide basic information for further research on the reproductive development of minifish.

关 键 词：DAZL DND Minifish Paedocypris progenetica 

分 类 号：S917.4[农业科学—水产科学]