姜黄素诱导沉默MDR1基因的SGC7901/ADM细胞凋亡  

Apoptosis of SGC7901/ADM Cells of Silencing MDR1 Gene Induced by Curcumin

作　　者：孙新迪[1] 邵淑丽[1] 李旭艳[1] 张伟伟[1] 张珍珠[1] 恽冬泽[1] 朱少伟[1] Sun Xindi;Shao Shuli;Li Xuyan;Zhang Weiwei;Zhang Zhenzhu;Yun Dongze;Zhu Shaowei(College of Life Sciences, Agriculture and Forestry, Qiqihar University, Qiqihar, 161006)

机构地区：[1]齐齐哈尔大学生命科学与农林学院

出　　处：《基因组学与应用生物学》2018年第7期3066-3070,共5页Genomics and Applied Biology

基　　金：黑龙江省自然科学基金(No.C201241;No.C200624;No.C2016057);黑龙江省教育厅科学技术项目(12511611);黑龙江省教育厅基本业务专项理工重点项目(135109104);齐齐哈尔大学研究生创新科研项目(YJSCX2016-ZD09)共同资助

摘　　要：为探讨MDR1基因沉默对姜黄素诱导人胃癌SGC7901/ADM细胞凋亡的影响,将已构建的靶向MDR1基因的RNAi表达载体转染SGC7901/ADM细胞,建立转染细胞单克隆,流式细胞术检测细胞外排功能。姜黄素处理SGC7901/ADM细胞48 h后,通过激光共聚焦显微镜下观察细胞形态结构,琼脂糖凝胶电泳检测DNA片段化。结果显示,各干扰载体转染组细胞内Rho-123荧光强度不同程度增强,细胞经姜黄素处理48 h后,激光共聚焦显微镜下呈明显的凋亡形态结构,DNA琼脂糖凝胶电泳呈梯状条带,说明MDR1基因沉默能促进姜黄素诱导SGC7901/ADM细胞凋亡。The aim of this study was to investigate the effects ofMDR1 gene silencing on the apoptosis of human gastric cancer SGC7901/ADM cells induced by curcumin. The constructed RNAi expression vector of targeting MDR1 gene was transfected into SGC7901/ADM cells, and one single clone was established, the cell effiux function was analyzed by flow cytometry. After the cells were treated with curcumin 48 hours, the morphological of the cells was observed under a confocal laser scanning microscope. The DNA fragmentation of the cells was evaluated by agarose gel electrophoresis. The results showed that fluorescence intensity of Rho-123 in the cells was transfected by various interference carriers was enhanced in different degrees. After treated with curcumin for 48 h, the apoptotic morphological structure was observed under laser confocal microscope, and DNA agarose gel electrophoresis showed a ladder band, which indicated that MDR1 gene silencing could promote the apoptosis of SGC7901/ADM induced by Curcumin.

关 键 词：SGC7901/ADM细胞 MDR1 姜黄素 

分 类 号：R285.5[医药卫生—中药学]