糯玉米品种‘沪玉糯3号’种子真实性与纯度鉴定的SSR核心引物筛选  

The application of SSR markers in the authenticity and purity assessment of glutinous maize variety ‘Huyunuo No. 3'

作　　者：卢媛[1] 韩晴[1] 王义发[1] 沈渊[2] 沈新芬[2] 瞿玉玑 施标[4] 沈雪芳[1] LU Yuan;HAN Qing;WANG Yi-fa;SHEN Yuan;SHEN Xin-fen;QU Yu-ji;SHI Biao;SHEN Xue-fang(Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;z Jinshan District Agro- Technology Extension Center, Shanghai 201599, China;Qingpu District ZhujiajiaoTown Agricultural Service Center, Shanghai 201713, China;Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]上海市金山区农业技术推广中心,上海201599 [3]上海市青浦区朱家角镇农业综合服务中心,上海201713 [4]上海市农业科学院,上海201403

出　　处：《上海农业学报》2018年第3期45-49,共5页Acta Agriculturae Shanghai

基　　金：上海市种业发展项目[沪农科种字(2016)第1-3号];上海市种业发展项目[沪农科种字(2013)第13号];上海市科学技术委员会科研计划项目(153919N1300);上海市市级农口系统青年人才成长计划[沪农青字(2015)第1-4]

摘　　要：利用40对玉米SSR核心引物对2个供试品种进行DNA指纹鉴定,确定‘沪玉糯3号’的真实性。通过在‘沪玉糯3号’及其双亲间扩增,发现SSR标记umc2007y4、bnlg1940k7、bnlg2305k4、bnlg1702k1、umc1125y3、phi080k15能在样品与‘沪玉糯3号’间扩增出清晰稳定的多态条带,因此,认为这6对SSR引物可用于‘沪玉糯3号’品种真实性的快速鉴定。此外,筛选出6对共显性SSR核心引物(bnlg1940k7、bnlg2305k4、umc1536k9、bnlg249k2、bnlg2235y5和phi233376y1),可用于‘沪玉糯3号’的纯度鉴定。SSR标记bnlg1940k7和bnlg2305k4作为首选,可同时对‘沪玉糯3号’种子进行真实性和纯度鉴定。为加强‘沪玉糯3号’的产权保护及种子质量监测提供了理论依据和技术支持。In order to determine the authenticity of glutinous maize variety ‘ Huyunuo No. 3 ’,2 testvarieties were assessed by DNA fingerprinting through 40 pairs of maize SSR core primers. After the amplificationbetween ‘ Huyunuo No. 3’ and its parents,6 pairs of SSR primers（umc2007 y4, bnlg1940 k7, bnlg2305 k4,bnlg1702 k1,umc1125 y3,phi080 k15） were found to be able to amplify clear and easily detectable polymorphicbands,based upon which,these 6 pairs of SSR primers were believed to be reliable markers for rapid authenticityassessment. Meanwhile,6 pairs of codominant SSR primers（bnlg1940 k7,bnlg2305 k4,umc1536 k9,bnlg249 k2,bnlg2235 y5 and phi233376 y1）were found to be effective for purity assessment. These 2 pairs of SSR primers,bnlg1940 k7 and bnlg2305 k4,which turned out to be useful for both authenticity and purity assessments,wereconsidered to be the most preferred markers for being cost-saving and highly efficient. This study providestheoretical basis for protecting the intellectual property of ‘ Huyunuo No. 3’ and offers technical support formonitoring the seed quality of the variety.

关 键 词：糯玉米 '沪玉糯3号’ 真实性 DNA指纹图谱 

分 类 号：S513[农业科学—作物学]