基于AHP-CRITIC法的正交设计优选参膝口服液提取工艺  

Optimization of extraction technique for Shenxi Oral Liquid by orthogonal design based on AHP-CRITIC analysis

作　　者：刘小妹 程中琴 施崇精 王姗姗 袁强华[2] 宋英[2] LIU Xiao-mei;CHENG Zhong-qin;SHI Chong-jing;WANG Shan-shan;YUAN Qiang-hua;SONG Ying(College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;Department of Pharmacy, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China)

机构地区：[1]成都中医药大学药学院,四川成都610075 [2]成都中医药大学附属医院药剂科,四川成都610072

出　　处：《中草药》2018年第11期2577-2583,共7页Chinese Traditional and Herbal Drugs

基　　金：四川省中医药管理局其他中医药支出(210069);国家中医药管理局国家中药标准化项目(ZYBZH-Y-SC-41)

摘　　要：目的确定指标权重,优选参膝口服液的最佳提取工艺。方法以HPLC法测定杯苋甾酮、人参皂苷Rg1、Re、Rb1的量,HPLC-ELSD法测定人参二醇的量、干膏率、总多糖含量及总峰面积作为评价指标,采用层次分析法(AHP)、基于指标相关性的权重确定方法(CRITIC)、AHP-CRITIC混合加权法确定各评价指标的权重系数,比较混合加权法和单一赋权法,并结合正交试验结果优选复方提取工艺的最佳参数。结果 AHP-CRITIC混合加权法较单一赋权法科学、合理、全面,按其所得的权重系数进行综合评价,优选出最佳工艺参数为复方药材加9倍量水,提取3次,每次80 min。3批验证试验综合评分均值为99.06,RSD为0.18%。结论 AHP法结合CRITIC法可用于参膝口服液提取工艺多指标权重的建立,优选的提取工艺经验证,合理、稳定,重复性好,可用于工业化生产。Objective The extraction process of Shenxi Oral Liquid by exploring the optional extraction process parameters based on determining the index weight. Methods Using the contents of cyasterone, ginsenosides Rg1, Re, Rb1, panaxadiol, ratio of dry extraction, total polysaccharides, and total peak area as comprehensive evaluation indexes, the weighting coefficient in eight synthetic evaluation indexes was determined by analytic hierarchy process（AHP）, criteria importance through intercriteria correlation（CRITIC） method, and mixed weighted AHP-CRITIC. Compared mixed weighting method and single weighting method, the optimal parameters of compound extraction process were optimized by orthogonal test. Results Mixed weighted AHP-CRITIC was more scientific, reasonable, and comprehensive than the single weighting method. According to the weighting coefficient determined by comprehensive evaluation, the optimal process parameters were as follow： compound herbs plus nine times of water, extracting three times, each for 80 min. The mean of three batches of comprehensive evaluation was 99.06 and RSD value was 0.18%. Conclusion AHP combined with CRITIC could be used to establish weighting coefficient of the compound formula extraction process and the optimized process of extraction had been verified to be reasonable, stable, and reproducible, which could be used for industrial production.

关 键 词：最佳工艺 指标 权重分析 AHP法 CRITIC法 AHP-CRITIC混合加权法 杯苋甾酮 人参皂苷Rg1 人参皂苷RE 人参皂苷Rb1 人参二醇 干膏率 总多糖 

分 类 号：R284.2[医药卫生—中药学]