稳定同位素示踪技术在内源性物质代谢调控中的应用进展  

Applications progress of stable isotopic tracer technique in metabolic regulation of endogenous substances

作　　者：令狐婷 刘少博 高耀[1,2] 史碧云 张翔 田俊生[1,2] 秦雪梅[1,2] LINGHU Ting;LIU Shao-bo;GAO Yao;SHI Bi-yun;ZHANG Xiang;TIAN Jun-sheng;QIN Xue-mei(Modem Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China;Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan 030006, China;College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;Departments of Chemistry, University of Louisville, KY 40292, USA)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]地产中药功效物质研究与利用山西省重点实验室,山西太原030006 [3]山西大学化学化工学院,山西太原030006 [4]路易斯维尔大学化学系,美国ky40292

出　　处：《中草药》2018年第11期2678-2685,共8页Chinese Traditional and Herbal Drugs

基　　金：国家"重大新药创制"科技重大专项(2017ZX09301047);山西省科技计划项目(201603D321077;201701D22111344);山西省科技创新重点团队(201605D131045-18);山西省重点实验室(201605D111004)

摘　　要：随着高灵敏度检测方法的快速发展,稳定同位素示踪技术受到越来越多的重视。该技术通过示踪原子追踪标记化合物在机体内的活动规律,依据中间代谢产物的同位素峰分布来判断其具体的代谢途径,通过计算通量对整个代谢通路进行综合分析,从而探究疾病的发生机制及药物代谢的途径和过程。近年来,稳定同位素示踪技术在生物医药领域的应用逐年增加,现主要针对其在糖、脂、氨基酸及激素等内源性物质代谢调控中的应用研究进展进行综述。With the rapid development of high-sensitivity detection methods, stable isotope tracing technique has received increasing attention. Stable isotope tracing technique can accurately track the activity of labeled compounds in the body through the tracer atoms and determine their specific metabolic pathways based on the distribution of isotopic peaks of the intermediate metabolites. By calculating the flux, the metabolic pathways are analyzed to provide a basis for the study of disease mechanism and drug metabolism. In recent years, the technique has a wide application in the field of biomedicine. This paper summarizes the applications of stable isotopic tracer technique in the metabolic regulation of endogenous substances such as carbohydrate metabolism, lipid metabolism, amino acid metabolism, hormone metabolism, nucleic acid metabolism, and so on.

关 键 词：稳定同位素 代谢流分析 内源性物质 代谢调控 代谢通路 

分 类 号：R285[医药卫生—中药学]