三七花及其易混淆品HPLC指纹图谱鉴别研究  

Identification of Notoginseng Flower and Its Adulterants by HPLC Fingerprint Chromatogram

作　　者：孟祥松 蒋磊 于现花 鞠康[1] 王伟 简冬明 穆二廷 周建理 MENG Xiangsong;JIANG Lei;YU Xianhua;JU Kang;WANG Wei;JIAN Dongming;MU Erting;ZHOU Jianli(Bozhou Vocational and Technical College, Bozhou 236800, Anhui, China;Bozhou Institute for Food and Drug Control, Bozhou 236800, Anhui, China;National Chinese Medicinal Materials Products Quality Supervision and Inspection Center, Bozhou 236800, Anhui, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, Anhui, China)

机构地区：[1]亳州职业技术学院,安徽亳州236800 [2]亳州市食品药品检验中心,安徽亳州236800 [3]国家中药材产品质量监督检验中心,安徽亳州236800 [4]安徽中医药大学药学院,安徽合肥230031

出　　处：《辽宁中医药大学学报》2018年第6期8-11,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：安徽省高等学校自然科学研究项目(KJ2017A776);安徽省高等学校省级质量工程项目(2017jxtd082)

摘　　要：目的：对三七花、人参花、西洋参花进行指纹图谱研究。方法：反相C18（250 mm×4.6 mm,5μm）色谱柱,乙腈-水梯度洗脱,检测波长203 nm;流速1.0 mL·min^-1;柱温：25℃;进样量：20μL;分析时间：95 min。结果：三种花的皂苷类成分均得到很好分离,构建了指纹图谱共有模式,并依据峰面积和保留时间进行了比较。西洋参花和三七花中皂苷Rb2和Rb3的峰面积比值有着很明显的差异,人参花中有独有峰Rg1,三七花中独有峰Rb1。结论：该方法简便、重复性好、特征性强,可用于鉴别三七花、人参花、西洋参花。Objective：The method was established to identify notoginseng flower,ginseng flower and american ginseng flower. Methods：RP-HPLC C18-analytical column（250 mm× 4.6 mm,5 μm）;acetonitrile-water as gradient eluent,flow rate 1.0 mL·min^-1,detective wavelength at 203 nm;column temperature：25 ℃;sample size：20 μL;analysis of time：95 min. Results：The fignerprints of notoginseng flower,ginseng flower and american ginseng flower were obtained,and all ginsenosides were analyzed perfectly. The common pattern of fingerprint was established,and the retention time and peak area were compared. The peak height ratio of ginseboside Rb2 and ginseboside Rb3 was a suitable character to differentiate the notoginseng flower and american ginseng flower. Ginseboside Rg1 as a unique peak of ginseng flower. Ginseboside Rb1 as a unique peak of notoginseng flower. Conclusion：The method is stable and reliable with a good reproducibility and provides a reference standard for identifying notoginseng flower and its adulterants.

关 键 词：三七花 人参花 西洋参花 HPLC指纹图谱 

分 类 号：R284.2[医药卫生—中药学]