多指标综合评分法正交试验优选肝乐颗粒醇提工艺  

Study on Alcohol Extraction Technology of Ganle Granule by Multi-index Comprehensive Scoring Method

作　　者：汪永忠[1] 柳清 潘凌宇[1,3] 韩燕全[1] 姜辉[1] WANG Yongzhong;LIU Qing;PAN Lingyu;HAN Yanquan;JIANG Hui(The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, Anhui, China;Huainan Vocational and Technical College, Huainan 232001, Anhui, China;Anhui University of Chinese Medicine, Hefei 230038, Anhui, China)

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031 [2]淮南职业技术学院,安徽淮南232001 [3]安徽中医药大学,安徽合肥230038

出　　处：《辽宁中医药大学学报》2018年第6期11-14,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：安徽省省级中医药发展专项项目(2015ZYZJ01)

摘　　要：目的：采用UPLC指纹图谱结合多指标综合评分法优选肝乐颗粒的醇提工艺。方法：采用正交设计法,以绿原酸和芍药苷的含量、干膏得率、正交样品指纹图谱中9个主要共有峰的总面积为指标,考察溶剂倍数、乙醇体积分数、提取时间和提取次数四因素对肝乐颗粒醇提工艺的影响。采用综合评分法进行数据分析,建立9个正交样品的指纹图谱,并对其用中国药典委员会颁布的中药色谱指纹图谱相似度评价系统软件（版本：2004A）和SPSS 19.0软件分别进行相似度分析、聚类分析,优选肝乐颗粒的最佳醇提工艺。结果：肝乐颗粒的最佳醇提工艺为：溶剂倍数为10倍量的60%乙醇回流提取3次,每次2 h;9个正交样品的相似度均大于0.97;聚类分析表明正交样品可大致聚成4类。结论：优选的提取工艺合理、可行,有效成分含量高,建立的指纹图谱重现性好,验证了优选醇提工艺的可行性,为肝乐颗粒醇提工艺的确定提供实验依据。Objective：To optimaize the alcohol extraction technique for Ganle Granule and confirm the optimum extraction process parameters by develop an UPLC fingerprint combined with multi index comprehensive weighted score evaluation. Methods：The orthogonal test was employed taking the contents of chlorogenic acid,paeoniflorin,dry extract rate and the total area of the 9 main peaks in the fingerprints of orthogonal samples as the indexes and the influences on extraction results of soaking time,solvent dosage and extraction times were investigated,and were analyzed by multi-index comprehensive scoring method. The fingerprint of the 9 orthogonal samples was established,and the chinese pharmacopoeia commission promulgated the chromatographic fingerprint similarity evaluation system software（version：2004 A）analyzed the similarity analysis,SPSS 19.0 software analyzed cluster analysis. Results：The optimal extracting process was selected as follows：10 times of alcohol consumption,60% ethanol reflux extraction three for three times,and two hour each time,the similarity of the 9 orthogonal samples was more than 0.97;the cluster analysis showed that the orthogonal samples can be roughly clustered into 4 categories. Conclution：The optimized extraction process is reasonable and feasible,and the effective component content is high,the fingerprint reproducibility is good,which verifies the feasibility of optimizing the alcohol extraction process,and provides the experimental basis for the determination of ethanol extraction process of the Ganle Granule.

关 键 词：多指标综合评分 正交试验 肝乐颗粒 提取工艺 绿原酸 芍药苷 

分 类 号：R284.2[医药卫生—中药学]