近红外光谱法快速测定升麻中皂苷含量  

Rapid Determination of Saponins in Cimicifugae Rhizoma by Near-infrared Spectroscopy Technique

作　　者：苏阳[1] 任文晨 于浩然[1] 祝清炜 孙野 吴伦[1] 匡海学[1] SU Yang;REN Wenchen;YU Haoran;ZHU Qingwei;SUN Ye;WU Lun;KUANG Haixue(Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang, China)

机构地区：[1]黑龙江中医药大学

出　　处：《辽宁中医药大学学报》2018年第6期36-40,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81603416);中国博士后科学基金面上资助项目(2016M600267);黑龙江省第七批博士后特别资助(2016年青年英才计划)项目(LBH-TZ1619);黑龙江省普通本科高等学校青年创新人才项目(UNPYSCT-2016207);哈尔滨市第三批科技计划(青年后备)项目

摘　　要：目的：建立快速测定升麻中升麻新苷I含量的近红外定量模型。方法：以高效液相色谱法测定升麻新苷Ⅰ的含量,运用近红外技术收集不同产地不同批次的升麻样品的光谱图,采用标准正态变换（SNV）加二阶导数（SD）的光谱预处理方法,通过偏最小二乘回归（PLS）法建立升麻中升麻新苷Ⅰ含量测定的近红外定量模型,并对已建立的定量模型进行验证。结果：建立的新苷Ⅰ校正模型的相关系数（R）为0.99077,校正均方差（RMSEC）为0.0123,用验证集进行模型验证,预测误差均方根（RMSEP）为0.0348。结论：结果表明该方法简便高效,建立的模型性能较好,对升麻中升麻新苷Ⅰ含量的预测准确可靠。Objective：To develop rapid determination models of cohosh new glucoside Ⅰ in Cimicifugae Rhizoma using near infrared（NIR）spectroscopy. Methods：HPLC method was used to determine the content of cohosh new glucoside Ⅰ,using near-infrared spectroscopy to collect samples of different regions and different batches,spectral preprocessing method using standard normal variate（SNV）plus second derivative（SD）,partial least-squares（PLS）method was used to create a quantitative model of Cimicifugae Rhizoma of cohosh new glucoside Ⅰ,and to verify the established quantitative model. Results：The correlation coefficient R was 0.99077,root mean square error of calibration（RMSEC）was 0.0123,validating the model with a validation set,root mean square error of prediction（RMSEP）was 0.0348. Conclusion：The method is simple and convenient,the quantitative model established in this study is good,accurate and reliable for the determination of the content of cohosh new glucoside Ⅰ in Cimicifugae Rhizoma.

关 键 词：升麻 升麻新苷Ⅰ 近红外光谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]