三清山黄杉林区土壤耐性孢子对花旗松生长的影响  

Effects of tolerance ectomycorrhizal spores on the growth of Pseudotsuga menziesii seedlings from soil collected from Chinese Douglas-fir forest in Sanqing mountain

作　　者：温祝桂 朱小梅[2] 刘冲[2] 赵宝泉[2] 邢锦城[2] 丁海荣[2] 董静[2] 洪立洲[2] WEN Zhugui;ZHU Xiaomei;LIU Chong;ZHAO Baoquan;XING Jincheng;DING Hairong;DONG Jing;HONG Lizhou(Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland, Yancheng Institute of Technology, Yancheng 224051, Jiangsu, China;Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, Jiangsu, China)

机构地区：[1]盐城工学院江苏省海洋滩涂生物化学与生物技术重点建设实验室,江苏盐城224051 [2]江苏沿海地区农业科学研究所,江苏盐城224002

出　　处：《中南林业科技大学学报》2018年第7期19-25,共7页Journal of Central South University of Forestry & Technology

基　　金：江苏省农业科技自主创新资金项目(CX(17)1004);江苏省自然科学基金(BK20170469);江苏省海洋滩涂生物化学与生物技术重点建设实验室开放基金(K2016-10)

摘　　要：对江西三清山黄杉林区耐性孢子与花旗松Pseudotsuga menziesii形成的菌根进行了研究,通过分析形成的菌根真菌种类、幼苗生物量以及土壤理化性质,研究了菌根数量、侵染率、不同种类对幼苗生长以及土壤微环境对菌根群落结构形成的影响。结果表明:外生菌根真菌的侵染可以有效促进宿主植物的生长,尤其是在两种菌种共存的情况下(地上:y=-0.010x2+0.042x+0.047,R2=0.308,P<0.001;地下:y=-0.007x2+0.032x+0.048,R2=0.225,P<0.001),其中菌种Meliniomyces sp.和Rhizopogon sp.对幼苗生长影响最为明显;菌根的侵染率与幼苗地上部生物量存在正相关性(y=0.054+0.145x,R2=0.404,P<0.001);在孢子群落中占优势地位,与宿主特异性共生,同时有效地促进宿主幼苗生长的菌种Rhizopogon sp.,可能是在黄杉林业重建中具有应用前景的菌根真菌;在所测定土壤因子中,K、OM、Cond以及p H值是影响菌根群落的主要因素。该研究结果为利用菌根技术进行林业重建提供了理论依据。Pseudotsuga menziesii seedlings were used to clarify the ectomycorrhizal（ECM） symbiosis formed by tolerance spores in soils collected from Sanqing Mountain, Jiangxi province. ECM species, inoculation rate, dry weight of seedlings and soil properties were tested in this study. Besides, the effects of ECM fungal number, inoculation rates and species in seedlings growth were analyzed. The results showed that inoculation with ECM fungi significantly improved the growth of seedlings, especially coexistence with 2 species（Shoots： y =-0.010 x^2 ＋ 0.042 x ＋ 0.047, R^2= 0.308, P〈0.001 and Roots： y =-0.007 x^2 ＋ 0.032 x ＋ 0.048, R^2= 0.225, P〈0.001）. The effects of Meliniomyces sp. and Rhizopogon sp. on the growth of seedlings were significantly. And there was a positive correlation between mycorrhizal colonization rates and shoots dry weight（y=0.054＋0.145 x, R^2=0.404, P〈0.001）. Dominance of Rhizopogon sp. in spore banks, potentially growth-promoting effects, and host specificity make it a promising ECM fungus, which could facilitate seedling establishment and its forest regeneration of the endangered Chinese Douglas-fir. Among the soil parameters we tested, K, OM, Cond and p H were the main factors affecting the mycorrhizal community. The results provided a theoretical basis for forestation by using mycorrhizal technology.

关 键 词：花旗松 菌根真菌 生长 林业重建 

分 类 号：S791.259[农业科学—林木遗传育种]