2001-2017年中国流行性腮腺炎病毒学监测及基因特征分析  

Viral surveillance and genetic characteristics of mumps viruses in China during 2001-2017

作　　者：崔爱利[1] 朱贞[1] 张燕[1] 邱琪[1] 毛乃颖[1] 许松涛[1] 姬奕昕[1] 王慧玲[1] 许文波[1] Cui Aili;Zhu Zhen;Zhang Yan;Qiu Qi;Mao Naiying;Xu Songtao;Ji Yixin;Wang Huiling;Xu Wenbo(WHO WPRO Regional Reference Measles/Rubella Laboratory and Key Laboratory of Medical Virology of National Health and Family Planning Commission, National Institute for Viral Disease Control and Pre- vention, Chinese Center for Disease Control and Prevention, Beijing 102206, China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所国家卫生和计划生育委员会医学病毒学和病毒病重点实验室世界卫生组织西太平洋区麻疹/风疹参比实验室

出　　处：《中国疫苗和免疫》2018年第3期249-255,共7页Chinese Journal of Vaccines and Immunization

基　　金：国家自然科学基金课题(81102170);“十二五”国家科技重大专项(2013ZXl0004-202;2012ZXl0004-201-003);行使WHO西太区麻疹风疹参比实验室职能(WPCHN1409379)

摘　　要：目的阐明中国大陆地区流行性腮腺炎（流腮）病毒学监测进展及腮腺炎病毒（MuV）基因特征。方法依托全国麻疹/风疹实验室网络,自2001年起在全国范围内开展流腮病毒学监测。通过病毒分离、Real-time RT-PCR和RT-PCR等方法对MuV进行实验室检测和病毒基因型别鉴定;依据SH靶基因316个核苷酸序列,分析中国大陆地区MuV基因型别及其分布特征。结果 2001-2017年从中国22个省份共获得MuV SH基因序列451条,通过MuV基因型鉴定,439条（97.33%）为F基因型,12条（2.67%）为G基因型;2001-2017年有多个MuV F基因型分支共循环;G基因型于2011-2016年在局部地区流行。结论中国大陆地区MuV优势流行株为F基因型,G基因型呈局部流行;需加强流腮的持续性病毒学监测。Objective To analyze progress of mumps virus surveillance and the genetic characteristics of mumps viruses（ MuV） circulating in China's Mainland. Methods We obtained mumps virus reports from the National Measles/Rubella Laboratory Network from 2001 to 2017. We detected,isolated,and genotyped MuV with real-time RT-PCR and RT-PCR,and analyzed the resulting MuV genotype distribution based on sequences of the 316 nucleotide SH gene. Results During 2001-2017,451 SH gene sequences were obtained from 22 provinces in China's Mainland. Genotyping showed that 439（ 97. 33%）sequences were genotype F and 12（ 2. 67%） were genotype G. Several branches of genotype F co-circulated from 2001 to 2017. Genotype G spread in local areas from 2011 to 2016. Conclusions Genotype F was the dominant genotype of MuV circulating in China's Mainland,while genotype G spread locally.Surveillance of mumps viruses should continue and should be strengthened.

关 键 词：腮腺炎病毒 病毒学监测 基因型 

分 类 号：R373.1[医药卫生—病原生物学] R373.6[医药卫生—基础医学]