淫羊藿苷对铁超载模型小鼠骨代谢的影响  

Effects of icariin on bone metabolism in iron overload mice

作　　者：何玉琼[1] 赵璐[1] 沈燚 韩婷[1] 张建花[3] 辛海量[1] 张巧艳[1] HE Yu-qiong;ZHAO Lu;SHEN Yi;HAN Ting;ZHANG Jian-hua;XIN Hai-liang;ZHANG Qiao-yan(School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Department of Pharmacy, Jiamusi University, Jiamusi 154007, China)

机构地区：[1]第二军医大学药学院,上海200433 [2]福建中医药大学药学院,福建福州350122 [3]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《药学学报》2018年第7期1100-1106,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金联合基金重点项目(U1505226)

摘　　要：探讨淫羊藿苷对铁超载致骨质疏松小鼠骨代谢的影响。采用腹腔注射右旋糖酐铁复制铁超载小鼠模型,将60只2月龄雄性C57/BL6小鼠按体重随机分为正常对照组、模型组、N-乙酰半胱氨酸(N-acetyl-L-cysteine,NAC)组及淫羊藿苷(icariin,ICA)低、中、高剂量(50、100、200 mg·kg^(-1))组。除正常对照组外,其余各组小鼠每周注射1次右旋糖酐铁(100 mg·kg^(-1))以建立铁超载模型,将NAC和ICA分别混悬于0.5%CMC-Na溶液中,按照体重给药,体积为10 m L·kg^(-1),每周给药6天。3个月后,用ELISA试剂盒检测小鼠血清的生化指标,微计算机断层扫描技术(micro computed tomography,Micro-CT)分析股骨远端骨密度和骨小梁微结构的变化,万能力学分析仪检测股骨的生物力学参数。与正常对照组相比,铁超载降低小鼠的骨密度,使小鼠骨组织的微结构和骨生物力学性能退化,增加小鼠血清铁和磷的含量,及抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase-5b,TRACP-5b)的活性,降低小鼠血清骨钙素(osteocalcin,OCN)的水平,降低小鼠肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)的活性,增加脂质过氧化物丙二醛(malondialdehyde,MDA)的含量。淫羊藿苷可增加铁超载小鼠的骨密度,改善骨小梁微结构的退化,提高股骨的力学性能,降低铁超载小鼠血清铁、磷含量及TRACP-5b和肝组织MDA活性,增加血清OCN的水平。以上结果提示淫羊藿苷通过抗氧化调控铁超载小鼠的骨代谢,减少骨丢失,改善骨组织微结构和生物力学性能。This study was designed to investigate the effects of icariin on bone metabolism in osteoprotic mice induced by iron overload, the model of iron overload mice was established by intraperitoneal injection of iron dextran（100 mg·kg^-1）. Sixty 2-month-old C57/BL6 male mice were randomly divided into six groups, including normal control group, model group, N-acetyl-L-cysteine（NAC）-treated group, icariin（50, 100 and 200 mg·kg^-1）-treated group. Except for the mice in control group, the mice were intraperitoneal injected weekly with iron dextran（100 mg·kg^-1） to establish the model of iron overload mice. The NAC and icariin were suspended in 0.5% CMC-Na solution, and administered orally for six times one week according to body weight. The mice in normal group and model group were given the same volume of 0.5% CMC-Na solution. Three months later, the organs, serum and femurs of mice were collected. Serum biochemical parameters were detected with an ELISA kit, the distal femur bone density and trabecular bone microstructure were analyzed by Micro-CT, and the mechanical properties of femur were measured by universal mechanical analyzer. Compared with the normal control group, iron overload decreased the bone mineral density and deteriorated the micro-architecture structure and bone mechanical properties in femur of mice, increased the level of iron, phosphorus and activity of tartrate resistant acid phosphatase-5b（TRACP-5b）, reduced the level of osteocalcin（OCN） in serum, decreased the activity of superoxide dismutase（SOD） of liver tissues, increased the content of malondialdehyde（MDA） of liver tissues. Icariin increased bone mineral density, improved the micro-architecture and mechanical properties of bone tissue, reduced the levels of iron and phosphorus, decreased the activity of TRACP-5b and enhanced the levels of OCN in serum, and also decreased the activity of MDA in liver tissue of iron overload mice. These results suggest that icariin is able to reduce bone loss and improve bone

关 键 词：淫羊藿苷 铁超载 骨代谢 右旋糖酐铁 

分 类 号：R965.1[医药卫生—药理学]