野生金针菇的分子鉴定及遗传多样性分析  

Molecular Identification and Genetic Diversity Analysis of Wild Flammulina sp.

作　　者：黄龙花[1] 谭武平 刘远超[1] 黄志勇 钟元茂 史钏 胡惠萍[1] HUANG Long-hua;TAN Wu-ping;LIU Yuan-chao;HUANG Zhi-yong;ZHONG Yuan-mao;SHI Chuan;HU Hui-ping(Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology South China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, China;Guangdong Yuewci Edible Fungi Technology Co. Ltd., Guangzhou 510663, China)

机构地区：[1]广东省微生物研究所,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东广州510070 [2]广东粤微食用菌技术有限公司,广东广州510663

出　　处：《中国食用菌》2018年第4期52-56,共5页Edible Fungi of China

基　　金：广东省科技计划项目(2017B020201007);广州市科技计划项目(201704020001).

摘　　要：针对采自全国7个自然保护区共计11个野生金针菇和2个常见栽培金针菇菌株,进行ITS-PCR和产物测序,经过与Gen Bank上已知序列的比对,结合传统形态特征鉴定,确定各菌株的拉丁名,并用MEGA6.0进行ITS序列的遗传距离分析和系统发育树构建。结果表明,金针菇的ITS1比ITS2进化速率快,变异位点和简约信息位点差异较大;13株金针菇种内遗传距离为0~0.014,种间遗传距离为0.029~0.045;ITS系统发育树也支持将13个菌株分为3个类群,即3个种,这与分子鉴定和传统形态鉴定结果相吻合,且同一菌种地域分布越近的菌株越容易聚为一支。研究表明,ITS序列分析可用于野生金针菇的分子鉴定和种间遗传多样性分析。In order to calculate the genetic distance between strains of Flammulina sp. and phylogenetie relationship of these strains, ITS sequences of eleven wild Flammulina sp. collected from the National Nature Preserve and two common cultivated Flammulina velutipes were amplified and sequenced. Genetic distance and phylogenetic relationship were analyzed by MEGA6.0. The results showed that the evolution rate of ITS1 is faster than ITS2, and the variable sites and parsimony informative sites of ITS1 differs greatly from ITS2. Intraspecific genetic distance of 13 experimental strains was 0-0.014, and the interspecific genetic distance of them was 0.029-0.045. Phylogenetic tree based on ITS sequences supported the view that 13 experimental strains interspecific could be divided into three broad categories, the closer of strains in regional relationship was also closer in phylogenetic relationship. The research indicated that ITS sequence analysis could be used for the molecular identification and genetic diversity analysis of wild Flammulina sp.

关 键 词：金针菇 ITS序列 系统发育树 遗传多样性 

分 类 号：S646.1[农业科学—蔬菜学]