基于体细胞胚发生的细叶百合和兰州百合多倍体诱导及鉴定  

Polyploidy Induction and Identification of Lilium pumilum and Lilium davidii var. unicolor Based on Somatic Embryogenesis

作　　者：孙红梅[1,2] 付麟岚 王志平 盖美竹 王春夏[1,2] SUN Hongme;FU Linlan;WANG Zhiping;GAI Meizhu;WANG Chunxia(College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China;Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, Shenyang 110866, China)

机构地区：[1]沈阳农业大学园艺学院,沈阳110866 [2]设施园艺省部共建教育部重点实验室,沈阳110866

出　　处：《园艺学报》2018年第6期1136-1146,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31471897);辽宁省高等学校优秀人才支持计划A类项目(LR2013029)

摘　　要：以细叶百合(Lilium pumilum DC.Fisch.)和兰州百合(Lilium davidii var.unicolor)的鳞片和胚性愈伤组织作为诱导材料,用不同浓度秋水仙素以浸泡和混培两种处理方式进行多倍体诱导。以形态学观测、根尖染色体计数、气孔观测对多倍体植株进行倍性鉴定,分别获得了19株细叶百合四倍体植株和6株兰州百合四倍体植株,最佳处理方式为0.1%秋水仙素浸泡胚性愈伤组织24 h。使用ISSR对经体细胞胚发生的二倍体和多倍体再生植株进行遗传分析,发现两种百合二倍体植株遗传较稳定,而多倍体植株均发生遗传变异,其中细叶百合和兰州百合遗传变异率分别为15.48%和9.75%。Using scales and embryogenic callus as the target tissues,Lilium pumilum DC. Fisch. and Lilium davidii var. unicolor were accomplished the ployploid inducement by soaking and mixed culture with different concentrations of colchicine. Nineteen tetraploids of Lilium pumilum DC. Fisch. and 6 tetraploids of Lilium davidii var. unicolor have been identified,through morphological observation,root tip chromosome count and stomatal observation. The optimum condition is immersing embryogenic callus with 0.1% colchicine for 24 hours to achieve the highest efficiency of chromosome doubling. Dipold plants of both lilies are genetically stable while polyploid plants experience the genetic variation by using ISSR to conduct the genetic analysis of plants via somatic embryogenesis. The variation rates of polyploid in Lilium pumilum DC. Fisch. and Lilium davidii var. unicolor are 15.48% and 9.75%,respectively.

关 键 词：细叶百合 兰州百合 体细胞胚发生 多倍体 ISSR 

分 类 号：S682.2[农业科学—观赏园艺]