内源CD133^＋细胞示踪小鼠模型的制备和鉴定  

Establishment and Identification of Endogenous CD133^＋ Cell Tracer Mouse Model

作　　者：韩明明 罗玉萍[1] HAN Ming-ming;LUO Yu-ping(School of Life Science, Nanchang University, Nanchang 330031, China)

机构地区：[1]南昌大学生命科学学院,南昌330031

出　　处：《中国生物工程杂志》2018年第6期58-62,共5页China Biotechnology

基　　金：国家自然科学基金（31660324）; 江西省自然科学基金重大项目（2015ACB20008）资助项目

摘　　要：目的：为了制备可追踪CD133阳性神经干细胞分化谱系的小鼠模型。方法：将两种C57B16背景的转基因小鼠CD133-Cre-ERT2和Rosa26-CAG-LSL-ZsGreen杂交,获得CD133-Cre ER;CAG-ZsGreen小鼠模型。结果：免疫组化和激光扫描共焦成像分析表明,经Tamoxifen作用后,该杂交小鼠在侧脑室SVZ区、第三脑室和第四脑室的室管膜区域均表达绿色荧光蛋白ZsGreen,且这些区域的绿色荧光与CD133＋红色荧光重合。结论：在室管膜区CD133＋是静息态神经干细胞的标志,因此,通过分析CD133-Cre ER;CAG-ZsGreen小鼠中的ZsGreen阳性细胞可追踪神经干细胞的细胞分化谱系。成功制备了内源CD133＋细胞示踪小鼠模型,为探讨大脑中CD133＋神经干细胞的激活、增殖、迁移和分化提供了帮助。Objective：To establish a mouse model that can trace the differentiation of CD133 positive neural stem cells.Methods：The CD133-Cre-ERT2 transgenic mice were mated with Rosa26-CAG-LSL-ZsGreen transgenic mice to produce CD133-Cre ER;CAG-ZsGreen double transgenic in C57B16 mice.Result：The immunohistochemistry and laser scanning confocal imaging analysis showed that Tamoxifen-Inducible Cre/lox P recombination,as depicted by green cells,existed in lateral ventricle SVZ,the ventricular zone of third ventricle and the fourth ventricle in adult mice,and the green fluorescence overlaps with CD133＋ red fluorescence in these regions.Conclusion：CD133＋ is the sign of the resting nerve stem cell in the ventricle membrane,so it can trace the cell differentiation lineages of neural stem cells by analyzing ZsGreen positive cells in CD133-Cre ER;CAG-ZsGreen mice.The mouse model for in vivo lineage tracing of endogenous CD133＋ cell was established successfully,which will be helpful to explore the activation,proliferation,migration and differentiation of CD133＋ neural stem cells in the brain.

关 键 词：CD133 神经干细胞 转基因小鼠 

分 类 号：Q78[生物学—分子生物学]