海岛棉GAUT基因家族的鉴定及其在棉纤维发育中的表达分析  

Genome-Wide Identification of GAUT Gene Family in Gossypium barbadense L. and Expression Analysis in Fiber Developmental Stages

作　　者：张松雨 刘正文 张艳[1] 杨君[1] 马峙英[1] 王省芬[1] ZHANG Song-yu;LIU Zheng-wen;ZHANG Yan;YANG Jun;MA Zhi-ying;WANG Xing-fen(North China Key Laboratory for Crop Germplasm Resources of Education, Ministry/Key Laboratory for Crop Germplasm Resources of Hebei/Hebei Agricultural University,Baoding 071000)

机构地区：[1]教育部华北作物种质资源重点实验室/河北省作物种质资源重点实验室/河北农业大学,保定071000

出　　处：《植物遗传资源学报》2018年第4期722-730,共9页Journal of Plant Genetic Resources

基　　金：国家转基因生物新品种培育科技重大专项(2016ZX08005-003);河北农业大学作物学科梯队建设基金(TD2016C201)

摘　　要：本研究鉴定了海岛棉全基因组中的GAUT基因家族,对其进行了生物信息学及纤维发育过程中的表达分析。结果表明,海岛棉GAUT家族包含37个成员,分为3个亚组,分布在海岛棉18条染色体上。GAUT氨基酸序列保守基序有4个,保守性较强,所有GAUT蛋白均定位在高尔基体膜。根据GAUTs在海岛棉纤维发育不同时期的表达变化,将其分为起始期高表达、纤维伸长期高表达、次生壁增厚期高表达、全时期低表达等4类模式。其中,GAUT05、GAUT06、GAUT07、GAUT23、GAUT24、GAUT26等在纤维发育某个时期具有优势表达的基因可能在纤维发育过程中发挥着重要作用。上述结果为研究海岛棉GAUT基因家族在棉纤维发育中的功能提供了参考依据。In this study the alpha-galacturonosyltransferase（ GAUT） family genes in the genome of Gossypium barbadense L. were identified and the expression analysis during the fiber development stages were carried out. The results showed that a total of 37 GAUT genes were identified from G. barbadense L. and classified into three subfamilies. They were distributed over 18 chromosomes of G. barbadense L. and had four conserved motifs. By predicting subcellular localization all GAUT proteins were located in the Golgi membrane. According to the transcriptional profiles of the GAUTs expression at the different stages of fiber development,they were divided into four expression patterns including high expression pattern of initial stage,high expression pattern of fiber elongation stage,high expression pattern of secondary wall thickening period and low expression pattern in the whole period. The differentially expressed genes GAUT05,GAUT06,GAUT07,GAUT23,GAUT24,GAUT26 might play important roles in fiber development. These results provided a useful reference for understanding the function of the GAUT gene family in the development of fiber in G. barbadense L..

关 键 词：海岛棉 半乳糖醛酸转移酶 生物信息学 纤维发育 基因表达 

分 类 号：S562[农业科学—作物学]