基于长尖叶蔷薇与大花香水月季转录组数据的MLO unigenes生物信息学及表达分析  

Bioinformatics and Expression Analysis of MLO-like genes Based on Rosa longicuspis and R. odorata var. gigantea Transcriptome Data

作　　者：向贵生 张颢[1,3] 王其刚 晏慧君[1,2,3] 周宁宁 陈敏[1,3] 唐开学 邱显钦[1,3] XIANG Gui- sheng;ZHANG Hao;WANG Qi- gang;YAN Hui-jun;ZHOU Ning- ning;CHEN Min;TANG Kai- xue;QIU Xian- qin(Flower Research Institute, Yunnan Academy of Agricultural Sciences ,Kunming 650205;School of Life Sciences, Yunnan University ,Kunming 650091;National Engineering Research Center for Ornamental Horticulture, Kunming 650205)

机构地区：[1]云南省农业科学院花卉研究所,昆明650205 [2]云南大学生命科学学院,昆明650091 [3]国家观赏园艺工程技术研究中心,昆明650205

出　　处：《植物遗传资源学报》2018年第4期769-779,共11页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31560565;31160403);国家科技支撑计划项目(2015BAD10B01);云南省应用基础研究计划项目(2014FB158);领军人才项目(2016HA005);云南省中青年学术技术带头人后备人才培养项目(2015HB078);云南省重大科技专项(2016ZA005)

摘　　要：在对长尖叶蔷薇和大花香水月季转录组测序的基础上,利用生物信息学方法,从其转录组数据中共获得23条MLO unigenes并对其进行了分析。结果显示它们之间的氨基酸数量、碱基数、分子量差异较小,多数为疏水性蛋白,平均亲水系数为-0.157~0.190,富含亮氨酸和丝氨酸。亚细胞定位主要分布于质膜中,包含5~9个跨膜结构;二级结构主要由α-螺旋组成。其中,等电点小于7的仅有1条,2条蛋白具有信号肽。这23条MLO蛋白具有15个长度在15~50个氨基酸间的保守基序。它们的系统进化关系分析结果揭示了MLO基因在长尖叶蔷薇与大花香水月季间具有较高的同源性和保守性。将这23条unigenes与来自于月季和拟南芥的MLO基因进行聚类分析,确定了6条可能参与抗白粉病的候选基因,对这6条候选基因进一步分析发现仅有2条unigenes符合MLO型白粉病基因的典型结构特征。最后,通过q RT-PCR试验验证,结果表明:这2条候选基因的相对表达量,在受到白粉病菌侵染后呈积极上调趋势。上述研究结果表明这2条候选基因很可能参与了寄主—白粉病菌的互作过程。Based on transcriptome sequencing of Rosa longicuspis Bertol. and R. odorata（ Andr.） Sweet var.gigantea（ Crep.） Rehd. et Wils.,23 MLO-likegenes were identified from the transcriptome data and analyzed by using bioinformatical methods. The results showed that between R. longicuspis Bertol. and R. odorata（ Andr.） Sweet var.gigantea（ Crep.） Rehd. et Wils.,the number of amino acids,base pairs and molecular weight of different proteins coded by genes did not vary significantly. Most of the protein were hydrophobicin with the average hydrophilic coefficients of-0. 157-0. 190. And these protein were rich in leucine and serine. By bioinformatics prediction,the deduced proteins of MLO-like genes were mostly located to the plasma membrane,with 5-9 predicted transmembrane motifs. Of that,the isoelectric point of one protein was less than 7. 0,and two proteins contained predicted signal peptides. These23 MLO proteins had 15 conserved motifs with length varying from 15 to 50 amino acids. The phylogenic analysis revealed that 23 MLO unigenes were highly homologous and conserved. By search for sequence homolog in Arabidopsisthaliana（ L.） Heynh.,six genes were identified to be associated with powdery mildew resistance in A. thaliana（ L.）Heynh.,two of which resembled the typical structure of MLO genes. q RT-PCR revealed up-regulation of these two genes was up-regulated post powdery mildew inoculation. Thus,this work suggested two MLO-like genes that possibly associated with powdery mildew resistance.

关 键 词：长尖叶蔷薇 大花香水月季 MLO基因 抗白粉病 生物信息学 QRT-PCR 

分 类 号：S685.12[农业科学—观赏园艺]