益气养阴通络方对糖尿病足小鼠胰岛素抵抗及胰岛β细胞的干预作用  

Effect of Yiqi Yangyin Tongluo Formula on Insulin Resistance and Islet β Cells of Diabetic Foot Mice

作　　者：宋莹莹[1] 张赓[1] 顾玲[1] 杨进[1] 王长明[1] SONG Yingying;ZHANG Geng;GU Ling;YANG Jin;WANG Changming(Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, China)

机构地区：[1]南京中医药大学

出　　处：《辽宁中医杂志》2018年第5期1083-1087,I0005,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81600966);江苏省自然科学基金项目(BK20161042);江苏省高校自然科学基金项目(16KJB320005);江苏省高校优势学科建设工程资助项目(PAPD)

摘　　要：目的：观察益气养阴通络方对糖尿病足（diabetic foot,DF）小鼠空腹血糖（fasting blood glucose,FBG）、空腹C-肽（fasting C-Peptide,FC-P）、空腹胰岛素（fasting insulin,FINS）、胰岛素样生长因子-1（insulin-like growth factor 1,IGF-1）表达变化,探讨益气养阴通络方对胰岛素抵抗、胰岛β细胞的干预作用。方法：SPF级C57BL/6J雄性小鼠70只,随机抽取10只为正常对照组,予普通饲料喂养,其余小鼠在给予高脂饮食喂养2周后,禁食16 h,腹腔快速注射STZ（streptozotocin,STZ）（120 mg/kg）造模,筛取符合糖尿病诊断标准的小鼠,用于建立DF模型,并按随机数字表分为6组：模型对照组、二甲双胍＋西洛他唑组（西药对照组）、通塞脉片组（中药对照组）、益气养阴通络方高、中、低剂量组。连续用药3周,观测各组小鼠的体质量、FBG、血清FC-P、FINS、IGF-1及胰岛素抵抗指数（homeostasis model assessment of insulin resistance index,HOMA-IR）的变化,并对胰岛进行免疫组织化学染色。结果：与模型对照组相比,益气养阴通络方高剂量组的体质量增加（P〈0.05）;高、中、低剂量组的FBG、HOMA-IR水平降低（P〈0.001）;高、中剂量组FC-P水平升高（P〈0.05或P〈0.001）;高剂量组IGF-1含量升高（P〈0.05）。与低剂量组比较,高、中剂量组的FINS含量增加（P〈0.05）;高剂量组FC-P含量增加（P〈0.01）。免疫组化染色及图像分析显示：益气养阴通络方能延缓小鼠胰岛萎缩,减轻胰岛细胞的病理损伤。结论：高剂量益气养阴通络方能有效地降低FBG,升高FC-P、IGF-1、FINS含量,降低胰岛素抵抗,提高胰岛素敏感性,促进胰岛结构及胰岛β细胞的修复,与西药对照组疗效比较接近,但高剂量组的综合效果优于中剂量组、低剂量组以及中药对照组。Objective： To observe the effect of Yiqi Yangyin Tongluo Formula on fasting blood glucose（FBG）,fasting C-Peptide（FC-P）,fasting insulin（FINS） and insulin-like growth factor 1（IGF-1） of diabetic foot（DF） mice and study the intervene of the formula on insulin resistance and islet β cells. Methods： Ten mice were randomly selected from 70 SPF male C57 BL/6 J mice and were fed by normal fodder. Other mice,fed by high fat fodder for 2 weeks,were given 120 mg/kg Streptozotocin（STZ）Injection once to establish models of type 2 diabetic mellitus after 16 hours fast. Successful DF models were divided into 6 groups according to random number table： model group,Metformin ＋ Clostazol group,Tongsaimai group,and high,middle and low dose of Yiqi Yangyin Tongluo Formula groups. During gavage for 3 weeks,body weights,FBG,FC-P,FINS,IGF-1 and homeostasis model assessment of insulin resistance index（HOMA-IR） were determined and pancreas was made immunohistochemical staining. Results： Compared with model group,body weight in high dose of Yiqi Yangyin Tongluo Formula group increased（P〈0. 05）. FBG and HOMA-IR in high,middle and low dose of Yiqi Yangyin Tongluo Formula groups decreased（P〈0. 001）. FC-P increased in high and middle dose groups（P〈0. 05 or P〈0. 001）,and IGF-1 increased in high dose group（P〈0. 05）. Compared with low dose group,FINS in high and middle dose groups increased（P〈0. 05） and FC-P in high dose group increased（P〈0. 01）. Immunohistochemical staining and image analysis displayed Yiqi Yangyin Tongluo Formula can delay pancreatic atrophy and reduce pathological damage of islet cells. Conclusion： High dose Yiqi Yangyin Tongluo Formula,similar to Metformin ＋ Clostazol,can lower FBG,raise FC-P,IGF-1 and FINS,decrease insulin resistance and enhance insulin sensitivity to promote islet structure recovery and islet β cell repair. These indexes above improve in high dose group more than those in middle dose group,low dose group or Tongsaimai group.

关 键 词：益气养阴通络 糖尿病足 胰岛素抵抗 胰岛Β细胞 

分 类 号：R587.2[医药卫生—内分泌]