长沙地区体检健康成年人血清脂蛋白相关磷脂酶A_2活性参考区间的建立  

Pilot study on reference interval of serum lipoprotein-associated phospholipase A2 activity in healthy adults of Changsha area,China

作　　者：廖琳 胡敏[1] 陈若虹[1] 喻丹 黄愉雯 谭雨纶 LIAO Lin;HU Min;CHEN Ruohong;YU Dan;HUANG Yuwen;TAN Yulun(Department of Clinical Laboratory, the Second Xiangya Hospital, Central South University, Changsha 410011;Xiangya School of Medicine, Central South University, Changsha 410001;Medicine School of Hunan Normal University, Changsha 410001, Hunan , China)

机构地区：[1]中南大学湘雅二医院检验科,长沙410011 [2]中南大学湘雅医学院,长沙410001 [3]湖南师范大学医学院,长沙410001

出　　处：《临床检验杂志》2018年第6期422-425,共4页Chinese Journal of Clinical Laboratory Science

摘　　要：目的调查长沙地区体检健康成年人血清脂蛋白相关磷脂酶A2(Lp-PLA2)活性水平,初步建立长沙地区血清Lp-PLA2活性的参考区间。方法选择424例体检健康成年人为研究对象,其中男175例,女249例,分别按年龄段20~29岁、30~39岁、40~49岁、50~59岁、60岁以上分为5组。用连续监测法检测血清Lp-PLA2活性,依据美国临床和实验室标准协会(CLSI)C28-A3要求,以非参数法建立血清Lp-PLA2活性的参考区间。结果男、女性血清Lp-PLA2活性均呈正态分布,男性Lp-PLA2为(478±135)U/L,女性为(402±116)U/L,差异有统计学意义(t=6.184,P<0.01)。经Z检验,男女两组Z>Z*,需按性别分组建立参考区间。男性各年龄段间差异无统计学意义(F=1.259,P=0.288)。女性各年龄段间差异有统计学意义(F=9.341,P<0.01);<40岁女性2个年龄组间(F=0.114,P=0.736)和>40岁女性3个年龄组间(F=1.873,P=0.158)差异无统计学意义,>40岁女性Lp-PLA2活性高于<40岁女性(t=5.732,P<0.01)。长沙地区体检健康成年人血清Lp-PLA2活性的参考区间男性为:217~761 U/L;女性为:168~566 U/L(20~39岁),203~702 U/L(40~86岁)。结论初步建立了长沙地区体检健康成年人血清Lp-PLA2活性的参考区间。Objective To investigate the level of serum lipoprotein-associated phospholipase A2（ Lp-PLA2） activity in healthy adults of Changsha area and establish the reference interval of serum Lp-PLA2 activity. Methods A total of 424 healthy adults（ 175 males and249 females） were classified into five groups by different age,including 20 to 29,30 to 39,40 to 49,50 to 59 and over 60 years old group. Serum Lp-PLA2 activity was measured by continuous-monitoring assay. According to the requirements of Clinical and Laboratory Standards Institute（ CLSI） C28-A3,the reference interval of Lp-PLA2 activity was established by nonparametric method. Results The levels of serum Lp-PLA2 activities in both males and females showed normal distribution. The average of Lp-PLA2 activity was（ 478±135） U/L in 175 males and（ 402±116） U/L in 249 females with statistically significant difference（ t = 6.184,P〈0.01）. Z test result showed ZZ＊,so the reference intervals of males and females were established respectively. There was no statistical difference of LpPLA2 activities among the varied groups of males（ F = 1.259,P = 0.288）,but there were statistical differences among the varied groups of females（ F = 9.341,P〈0.01）. The females of the age over 40 years old showed higher activities than those of age under 40 years old（ t= 5.732,P〈0.01）. However,there was no statistical significance of serum Lp-PLA2 activities in the females between the two groups of the age under 40 and the three groups of age over 40. Therefore,the reference intervals of serum Lp-PLA2 activities in healthy adults were established as followed： 217-761 U/L for males,168-566 U/L for the females of 20 to 39 years old and 203-702 U/L for the females of 40 to 86 years old. Conclusion The reference interval of serum Lp-PLA2 activity in physical examination of healthy adults in Changsha area was established.

关 键 词：脂蛋白相关磷脂酶A2 参考区间 心脑血管疾病 

分 类 号：R446.1[医药卫生—诊断学]