厚藤ASR基因克隆及功能初步分析  

Isolation and functional characterization of the ASR gene from Ipomoea pes-caprae

作　　者：张会[1,2] 郑洁旋 简曙光 夏快飞[1] 张美[1] Zhang Hui;Zheng Jie-Xuan;Jian Shu-Guang;Xia Kuai-Fei;Zhang Mei(Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院华南植物园,广东省应用植物学重点实验室,广州510650 [2]中国科学院大学,北京100049

出　　处：《植物科学学报》2018年第3期402-410,共9页Plant Science Journal

基　　金：国家重点研发计划项目(2016YFC1403002); 中国科学院A类战略性先导科技专项(XDA13020500); “十二五”农村领域国家科技计划项目(2015BAL04B04)

摘　　要：通过对厚藤(Ipomoea pes-caprae(Linn.)Sweet.)cDNA文库的筛选,获得了一个编码厚藤ASR(ABA-stress-ripening)基因的全长cDNA,命名为IpASR。研究结果显示,IpASR编码区全长648 bp,共编码215个氨基酸;蛋白质等电点为5.42,分子量为24.57 k D。通过在酵母中表达,发现IpASR能够提高转基因酵母的耐盐性及抗氧化能力。进一步以厚藤成年植株及幼苗为材料进行实时荧光定量PCR分析,结果表明,IpASR基因在厚藤成年植株各组织中广泛表达;高盐、甘露醇胁迫和ABA处理可诱导该基因在厚藤幼苗中的表达。结合GFP融合蛋白的亚细胞定位和生物信息学分析,发现IpASR蛋白为核蛋白,推测IpASR基因参与了厚藤生长发育的调控,并可响应ABA和非生物胁迫的诱导。We focused on the full-length cDNA encoding ASR protein isolated from a Ipomoea pes-caprae（ Linn.） Sweet. c DNA library. Results showed the coding region of IpASR cDNA was 648 bp,encoding a 215 amino acid protein with a molecular weight of 24. 57 kD and isoelectric point of 5. 42. By ectopic expression of IpASR in yeast,we found that IpASR improved the salt and H_2O_2 tolerance of transgenic yeast strains. Using adult plants and seedlings of I. pes-caprae with or without abiotic stress and ABA treatment,real-time RT-PCR analysis showed that IpASR was widely expressed in different adult organs in I. pes-caprae.The IpASR transcript was induced under abiotic stress and ABA treatment. The subcellular localization assay combining bioinformatics analysis showed that IpASR was a nucleoprotein.These results suggest that IpASR might play an important role in the regulation of I. pes-caprae growth and development,and therefore respond to environmental abiotic stress and the ABA signal pathway.

关 键 词：厚藤 ASR基因 逆境胁迫 亚细胞定位 

分 类 号：Q943.2[生物学—植物学]