囊状黄丝藻在不同初始氮浓度条件下特殊的油脂积累规律  

Novel lipid accumulation pattern in a filamentous oleaginous microalga Tribonema utriculosum SAG22.94 under different initial nitrogen concentrations

作　　者：徐梓钧 胡强 刘国祥[4] 胡征宇[4] 张婷[5] 张成武[1] Xu Zi-Jun;Hu Qiang;Liu Guo-Xiang;Hu Zheng-Yu;Zhang Ting;Zhang Cheng-Wu(Research Center of Hydrobiology, Department of Ecology, Jinan University, Guangzhou 510632, China;SDIC Microalgae Biotechnology Center, SDIC Biotechnology Investment Co. Ltd., Beijing 100000, China;Beijing Key Laboratory of Algae Biomass, Beijing 100000;Institute of Hydrobiology, Chinese Academy of Sciences, State Key Laboratory of Freshwater Ecology ＆ Biotechnology, Wuhan 430072, China;Life Science and Technology College, Xinjiang University, Urumqi 830046, China)

机构地区：[1]暨南大学生态学系水生生物研究中心,广州510632 [2]国投生物科技投资有限公司,微藻生物科技中心,北京100000 [3]微藻生物能源与资源北京市重点实验室,北京100000 [4]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,武汉430072 [5]新疆大学,生命科学与技术学院,乌鲁木齐830046

出　　处：《植物科学学报》2018年第3期411-419,共9页Plant Science Journal

基　　金：国投生物科技投资有限公司项目(SDICMBC-00003); 国家高技术研究发展计划“863”项目(2013AA065805); 国家自然科学基金项目(31170337); 广东省低碳专项(2011-051); 珠海市科技重大项目(PB20041018)

摘　　要：对不同初始氮浓度条件下囊状黄丝藻(Tribonema utriculosum SAG22.94)的生长状况、油脂含量和脂肪酸组成与含量进行研究。结果显示,囊状黄丝藻在氮浓度为3.0 mmol/L时,获得生物质浓度最高,为6.39 g/L;氮浓度为18.0 mmol/L时获得总脂和总脂肪酸含量最高,分别为细胞干重的44.62%和42.21%;上述3个指标单位体积的产率均在氮浓度3.0 mmol/L时达到最高值,分别为0.538、0.209和0.206 g·L^(-1)·d^(-1)。在4种初始氮浓度条件下,囊状黄丝藻油脂和脂肪酸含量可随着氮浓度增加而增加。脂肪酸含量分析结果显示,该藻的主要脂肪酸为豆蔻酸(C14∶0)、棕榈酸(C16∶0)、棕榈油酸(C16∶1ω7)、花生四烯酸(C20∶4ω6)和二十碳五烯酸(C20∶5ω3,EPA)。其中棕榈油酸含量最高,占总脂肪酸含量的36.53%~50.08%。研究结果表明囊状黄丝藻在不同初始氮浓度条件下具有特殊的油脂积累规律,是一株具有重要应用价值的产油丝状微藻。We investigated the effects on growth,lipid content,and fatty acid profiles of Tribonema utriculosum SAG22. 94 cultured under four different initial nitrogen concentrations（ 18.0,3.0,1.0,and 0 mmol/L）. Results showed that the unbranched filaments were composed of cylindrical cells 9～（-1）5 μm wide and 17-29 μm long. The highest biomassconcentration of T. utriculosum SAG22. 94 cultured at a nitrogen concentration of 3. 0 mmol/L was 6. 39 g/L. The highest total lipid and total fatty acid contents were 44. 62% and 42. 21% of dry cell weight,respectively,when the filamentous microalgae were cultured under a nitrogen concentration of 18. 0 mmol/L. The highest biomass and lipid and fatty acid productivities of0. 538,0. 209, and 0. 206 g · L^-1·d^-1 were obtained under a nitrogen concentration of3. 0 mmol/L. Interestingly,total lipid and total fatty acid contents increased with the increase in nitrogen concentration within a certain threshold. In addition,the main fatty acid components of Tribonema utriculosum SAG22. 94 were myristic acid（ C14 ∶ 0）,palmitic acid（ C16 ∶ 0）,palmitoleic acid（ C16 ∶ 1ω7）,arachidonic acid（ C20 ∶ 4ω6）,and eicosapentaenoic acid（ C20 ∶ 5ω3,EPA）. Among them,palmitoleic acid（ C16 ∶ 1ω7） was the dominant fatty acid and accounted for 36. 53%-50. 08% of total fatty acids. In short,T. utriculosum SAG22. 94 is a promising oleaginous filamentous microalga,with a unique lipid accumulation pattern.

关 键 词：囊状黄丝藻 氮浓度 总脂 脂肪酸 

分 类 号：Q949.2[生物学—植物学]