HPLC法同时测定化瘀祛斑胶囊中芍药苷、黄芩苷和黄芩素的含量  

Determination of Paeoniflorin,Baicalin and Baicalein in Huayuquban Capsules by HPLC

作　　者：黎春彤[1] 段斯庭 李翔[1] 刘皈阳[1] 马建丽[1] Li Chuntong;Duan Siting;Li Xiang;Liu Guiyang;Ma Jianli(Department of Pharmacy, the First Affiliated Hospital of PLA General Hospital, Beijing 100048, China)

机构地区：[1]解放军总医院第一附属医院药剂药理科,北京100048

出　　处：《中国药师》2018年第7期1287-1289,共3页China Pharmacist

基　　金：军队医疗机构制剂标准提高科研专项课题(编号:14ZJZ20-3)

摘　　要：目的:建立高效液相色谱法同时测定化瘀祛斑胶囊中芍药苷、黄芩苷和黄芩素的含量。方法:色谱柱为Agilent Zorbax SB-C_(18)柱(150 mm×4.6 mm,5μm);流动相为甲醇-0.1%磷酸溶液,梯度洗脱;流速为1.0 ml·min^(-1);检测波长为230 nm(0~15 min),277 nm(15~45 min);柱温为25℃;进样量为10μl。结果:芍药苷、黄芩苷和黄芩素的线性范围分别为1.295~25.890μg·ml^(-1)(r=0.999 9)、30.050~601.000μg·ml^(-1)(r=0.999 9)、1.874~37.480μg·ml^(-1)(r=0.999 9),平均回收率分别为100.9%,100.3%,99.31%,RSD分别为0.92%,1.30%,0.89%。结论:该法简便、快捷、结果准确、重复性好、实用性强,可以用于化瘀祛斑胶囊的质量控制。Objective： To establish an HPLC method for determining the contents of paeoniflorin,baicalin and baicalein in Huayuquban capsules. Methods： The separation was carried out on an Agilent Zorbax SB-C18（ 150 mm × 4. 6 mm,5 μm） column. The mobile phase consisted of 0. 1% phosphoric acid（ A） and methanol（ B） with gradient elution. The flow rate was 1. 0 ml·min^-1; the detection wavelengths were set at 230 nm（ 0-15 min） and 277 nm（ 15-45 min）. The column temperature was 25 ℃ with the injection volume of 10 μl. Results： The linear range was 1. 295-25. 890 μg·ml^-1（ r = 0. 999 9） for paeoniflorin,30. 050-601. 000 μg·ml^-1（ r = 0. 999 9） for baicalin,and 1. 874-37. 480 μg·ml^-1（ r = 0. 999 9） for baicalein. The average recovery was 100. 9%（ RSD =0. 92%） for paeoniflorin,100. 3%（ RSD = 1. 30%） for baicalin and 99. 31%（ RSD = 0. 89%） for baicalein. Conclusion： The method is rapid,convenient,accurate and stable,and suitable for the quality control of Huayuquban capsules.

关 键 词：化瘀祛斑胶囊 含量测定 芍药苷 黄芩苷 黄芩素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]