气相色谱法测定酮洛芬凝胶中乙醇含量  

Determination of Ethanol in Ketoprofen Gel by Gas Chromatography

作　　者：卢山 曹艳 肖学成 Lu Shah;Cao Yan;Xiao Xuecheng(College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, Chin)

机构地区：[1]湖北中医药大学药学院,武汉430065

出　　处：《中国药师》2018年第7期1309-1311,共3页China Pharmacist

摘　　要：目的:建立酮洛芬凝胶中乙醇含量的气相色谱测定方法。方法:色谱柱为Agilent DB-624(60 m×0.53 mm,3.00μm)毛细管柱,起始温度为40℃,维持2 min,以每min 3℃的速率升温至65℃,再以每min 25℃的速率升温至200℃,维持10 min;进样口温度200℃;检测器(FID)温度220℃;采用顶空分流进样,分流比为1∶1;顶空瓶平衡温度为85℃,平衡时间为20 min。结果:乙醇浓度在0.079~0.790 mg·ml^(-1)范围内呈良好的线性关系(r=0.999 8),平均回收率为99.8%(RSD=0.77%,n=9)。结论:本方法操作简便,结果准确,重复性好,可用于酮洛芬凝胶中乙醇含量的测定。Objective： To establish a GC method for the determination of ethanol in ketoprofen gel. Methods： The chromatographic capillary column was Agilent DB-624（ 60 m × 0. 53 mm,3. 00 μm） column. The programmed temperature was started at 40℃,maintained for 2 min,and than rose to 65℃ at a rate of 3℃ ·min^-1,and finally rose to 200℃ at a rate of 25℃ ·min^-1,maintained for 10 min. The temperature of the injection inlet was 200℃,and the FID detection was set at 220℃. Headspace split injection was used with the split ratio of 1： 1. The equilibrium temperature of headspace bottle was set at 85℃ with equilibration time of 20 min. Results： A good linear relationship within the concentration range from 0. 079 mg·ml^-1 to 0. 790 mg·ml^-1（ r = 0. 999 8） was obtained for ethanol,and the average recovery was 99. 8%（ RSD = 0. 77%,n = 9）. Conclusion： The method is simple,accurate and reproducible in the determination of ethanol in ketoprofen gel.

关 键 词：酮洛芬凝胶 乙醇 含量 气相色谱法 

分 类 号：R927.2[医药卫生—药学]