鸡VLDLR基因在不同组织中的表达分析  

Analysis of VLDLR expression in different tissues in chicken

作　　者：魏瑞 王聪[1] 杨荔 吴静怡 张汤杰[1] WEI Rui;WANG Cong;YANG li;WU Jingyi;ZHANG Tangjie(College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Chin)

机构地区：[1]扬州大学兽医学院

出　　处：《畜牧与兽医》2018年第7期60-63,共4页Animal Husbandry & Veterinary Medicine

基　　金：江苏省动物重要疫病与人兽共患病防控协同创新中心项目;江苏高校优势学科建设工程资助项目;扬州大学大学生科创基金重点项目

摘　　要：极低密度脂蛋白受体（VLDLR）是低密度脂蛋白受体家族的一员,能以高亲和力结合富含Apo E的脂蛋白,是机体细胞摄取血液中富含甘油三酯的极低密度脂蛋白（VLDL）的主要受体。本研究旨在探讨VLDLR基因在鸡不同组织内的表达水平和分布情况,为进一步研究VLDLR在肿瘤发生中的变化提供依据。采集成年鸡骨骼肌、肺脏、脂肪、脾脏、肝脏等组织,提取RNA,根据Gen Bank中的鸡VLDLR基因序列设计引物并进行RT-PCR扩增,检测鸡5种组织中VLDLR基因的相对表达水平。结果显示VLDLR基因在鸡不同组织间的表达存在差异,表现为肌肉表达量最高,肺脏和脂肪组织次之,肝脏表达量最低。相对表达量由高到低依次为骨骼肌（10.95±0.14）、肺脏（3.87±0.23）、脂肪组织（3.02±0.11）、脾脏（2.31±0.08）和肝脏（1.00±0.17）。本试验证实VLDLR基因在肺脏有较高的表达量,脾脏也有一定的表达。The very low density lipoprotein receptor（ VLDLR） is a member of the low-density lipoprotein receptor family. It possesses high affinity in binding Apo E-rich lipoprotein and is the main receptor for uptake of blood triglyceride-rich very-low-density lipid protein（ VLDL） in the body cells. This study was to investigate the expression level and distribution of the VLDLR gene in different tissues of chickens,and to provide evidence for the further study of VLDLR expression in tumorigenesis. Samples of lung,fat,spleen,liver and other tissues were collected quickly and stored in liquid nitrogen for RNA extraction. The expression of VLDLR in the five tissues of chickens was analyzed by designing primers based on chicken VLDLR sequence in Gen Bank and by performing RT-PCR amplification. The relative expression levels of VLDLR in the five tissues were detected. The results showed that the expressions of the VLDLR gene were different among the tissue samples： the highest in muscle,followed by lung and adipose tissue,and the lowest in liver. The relative expressions were that in skeletal muscle（ 10. 95 ± 0. 14） 〉that in lung（ 3. 87±0. 23）〉 that in adipose（ 3. 02±0. 11） 〉that in spleen（ 2. 31±0. 08） 〉that in liver（ 1. 00±0. 17）.To sum up,the VLDLR gene in the lungs had a higher expression,and that in the spleen had expression to a certain extent.

关 键 词：VLDLR基因 MRNA 实时荧光定量PCR 鸡 

分 类 号：S852.2[农业科学—基础兽医学]